Orta T, Eady J J, Peacock J H, Steel G G
Radiotherapy Research Unit, Institute of Cancer Research, Sutton, UK.
Int J Radiat Biol. 1995 Oct;68(4):413-9. doi: 10.1080/09553009514551371.
We have studied the role of glutathione (GSH) in determining radiation response in five human tumour and one human fibroblast cell line. GSH concentration was measured using the Tietze assay and compared with clonogenic survival following gamma-irradiation. No relationship between GSH concentration and aerobic radiosensitivity was observed. The addition of 10 mM extracellular cysteamine produced protection factors in all cell lines, ranging from 1.6 to 2.1, but had little influence on cellular GSH concentration. Depletion of GSH by buthionine sulphoximine (0.1 mM for 18 h) had negligible effect on cell survival, though moderate radiosensitization resulted from extreme GSH depletion after 30-min treatment with 1 mM dimethylfumarate. The degree of aerobic sensitization did not correlate with GSH levels. Irradiation under hypoxia produced oxygen enhancement ratios varying from 1.6 to 2.6, with no relationship to GSH content.
我们研究了谷胱甘肽（GSH）在五种人类肿瘤细胞系和一种人类成纤维细胞系中对辐射反应的影响。使用蒂茨测定法测量GSH浓度，并与γ射线照射后的克隆形成存活率进行比较。未观察到GSH浓度与需氧放射敏感性之间的关系。添加10 mM细胞外半胱胺在所有细胞系中产生了1.6至2.1的保护因子，但对细胞GSH浓度影响很小。用丁硫氨酸亚砜亚胺（0.1 mM，处理18小时）耗尽GSH对细胞存活的影响可忽略不计，尽管在用1 mM富马酸二甲酯处理30分钟后，极端的GSH耗竭导致了中度放射增敏作用。需氧增敏程度与GSH水平无关。缺氧条件下照射产生的氧增强比在1.6至2.6之间，与GSH含量无关。
