Hughes D P, Crispe I N
Immunobiology Section, Yale University Medical School, New Haven, Connecticut 06510, USA.
J Exp Med. 1995 Nov 1;182(5):1395-401. doi: 10.1084/jem.182.5.1395.
We report a soluble isoform of mouse Fas, which is generated by alternative splicing of Fas mRNA to a newly identified exon located between exons 2 and 3 of the previously published Fas sequence. This splicing event creates a novel Fas transcript, Fas beta, with the potential to encode a truncated form of the extracellular domain, termed Fas B. In vitro, P815 mastocytoma cells transfected with Fas B become resistant to Fas ligand-induced apoptosis, and the resistance is mediated by a secreted product of the transfected cells. In vivo, Fas beta mRNA expression is correlated inversely with apoptosis among subsets of intrahepatic T lymphocytes, a cell population in which activation-induced T cell apoptosis occurs. We propose that Fas B is a new cytokine that acts physiologically to limit apoptosis induced by Fas ligand.
我们报道了小鼠Fas的一种可溶性异构体，它是通过Fas mRNA选择性剪接至一个新鉴定的外显子产生的，该外显子位于先前发表的Fas序列的外显子2和3之间。这种剪接事件产生了一种新的Fas转录本Fasβ，它有可能编码一种截短形式的细胞外结构域，称为Fas B。在体外，用Fas B转染的P815肥大细胞瘤细胞对Fas配体诱导的凋亡产生抗性，并且这种抗性由转染细胞的分泌产物介导。在体内，Fasβ mRNA表达与肝内T淋巴细胞亚群中的凋亡呈负相关，肝内T淋巴细胞是一个发生激活诱导的T细胞凋亡的细胞群体。我们提出Fas B是一种新的细胞因子，其在生理上起作用以限制Fas配体诱导的凋亡。
