Chan W Y, Ng T B
Department of Anatomy (W.Y.C.), Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
J Exp Zool. 1995 Oct 1;273(2):160-9. doi: 10.1002/jez.1402730209.
The effect of hypothyroidism induced by 6-propyl-2-thiouracil (PTU) or thiourea (TU) on the development of the reproductive system in male and female neonatal ICR mice was investigated. PTU or TU was injected subcutaneously into experimental animals from postnatal day 1 (PD1) onward. The histological changes of the reproductive organs, formation of ovarian follicles, and spermatogenesis were examined on PD 14, 21, and 28, and the fertility of the hypothyroid mice in adulthood was followed. It was found that PTU or TU treatment did not produce an effect on the histology of the neonatal uterus and oviduct. In contrast, the drugs induced a decrease in the number of primordial follicles, multilaminar follicles, and Graafian follicles in the ovary. The number of follicles with degenerated follicular cells was increased. In the testis both PTU and TU treatments brought about a decrease in the number of seminiferous tubules with developing spermatids although the mean diameter of seminiferous tubules and the histology of the testis, epididymis, seminal vesicle, and coagulating gland was unaffected. The mating between hypothyroid females and euthyroid males and that between hypothyroid males and euthyroid females were normal with regard to the pregnancy rate, litter size, and sex ratio of offspring. The somatic growth of the resulting offspring was normal. It is concluded that the retarding effect on ovarian and testicular development in mice during neonatal period was not serious enough to adversely affect reproduction in the hypothyroid animals.
研究了6-丙基-2-硫氧嘧啶（PTU）或硫脲（TU）诱导的甲状腺功能减退对新生ICR雄性和雌性小鼠生殖系统发育的影响。从出生后第1天（PD1）起，将PTU或TU皮下注射到实验动物体内。在PD 14、21和28检查生殖器官的组织学变化、卵巢卵泡的形成和精子发生，并跟踪成年甲状腺功能减退小鼠的生育能力。发现PTU或TU处理对新生小鼠子宫和输卵管的组织学没有影响。相反，这些药物导致卵巢中原始卵泡、多层卵泡和格拉夫卵泡数量减少。卵泡细胞退化的卵泡数量增加。在睾丸中，PTU和TU处理均导致含有发育中精子细胞的生精小管数量减少，尽管生精小管的平均直径以及睾丸、附睾、精囊和凝固腺的组织学未受影响。甲状腺功能减退的雌性与甲状腺功能正常的雄性之间以及甲状腺功能减退的雄性与甲状腺功能正常的雌性之间的交配在妊娠率、窝仔数和后代性别比例方面均正常。所产后代的身体生长正常。得出的结论是，新生儿期对小鼠卵巢和睾丸发育的延缓作用不够严重，不足以对甲状腺功能减退动物的繁殖产生不利影响。
