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Gender identification of dried human bloodstains using fluorescence in situ hybridization.

作者信息

Pettenati M J, Rao P N, Schnell S, Hayworth-Hodge R, Lantz P E, Geisinger K R

机构信息

Department of Pediatrics, Bowman Gray School of Medicine of Wake Forest University, USA.

出版信息

J Forensic Sci. 1995 Sep;40(5):885-7.

PMID:7595332
Abstract

Identification of the gender of an individual(s) from whom a bloodstain is derived represents important evidence in medicolegal cases. The efficacy of fluorescence in situ hybridization (FISH) using chromosome X and Y centromeric probes was tested to determine its ability to identify correctly the gender of extracted dried bloodstains. In this preliminary study, FISH correctly identified the gender of 2-week-old dried bloodstains in prepared mixtures of male-to-female blood as low as 1%. The technique is accurate, rapid, sensitive, easily performed and readily available. This application of FISH as a forensic laboratory technique holds great promise.

摘要

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引用本文的文献

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Isolating cells from female/male blood mixtures using florescence in situ hybridization combined with low volume PCR and its application in forensic science.利用荧光原位杂交结合微量聚合酶链反应从女性/男性血液混合物中分离细胞及其在法医学中的应用。
Int J Legal Med. 2015 Nov;129(6):1211-5. doi: 10.1007/s00414-014-1103-0. Epub 2014 Nov 14.
2
Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures.悬浮荧光原位杂交(S-FISH)结合自动检测和激光显微切割技术,对男性/女性混合样本中的男性细胞进行 STR 分析。
Int J Legal Med. 2009 Sep;123(5):441-7. doi: 10.1007/s00414-009-0341-z. Epub 2009 Mar 25.