Pernthaler H, Eigentler A, Pfurtscheller G, Plattner R, Ofner D, Klima G, Kreczy A, Saltuari L, Margreiter R
Department of Transplant Surgery, University Hospital, Innsbruck, Austria.
J Pediatr Surg. 1995 Apr;30(4):573-6. doi: 10.1016/0022-3468(95)90134-5.
The importance of phase III of the migrating myoelectric complex (MMC) for homeostasis of enteric flora is well documented. The goal of this study was to evaluate in an isogeneic rat model the effect of MMC changes on the self-purging capacity of the jejunal graft. The proximal 25% of the entire jejunoileum of Lewis rats was transplanted orthotopically. Electrodes were then fixed to the graft. Native bowel of five rats and five rats with analogue jejunal segmentation served as controls. Myoelectric recordings were carried out until day 21, when animals were killed for bacteriologic analysis of the segments analyzed myoelectrically and the of neighboring gut. MMCs were observed in all animals during all recordings. Phase III was irregular in transplants because of long-lasting periods of phase III absence alternating with phase III occurring more frequently. The variation coefficient of phase III periodicity calculated for grafts was 48.74, for native bowel 14.79, and for segmented jejunum 22.9. Enteric flora found in all specimens consisted of colonic-like microorganisms. Titers of microorganisms in grafts did not differ from control segments. These findings show that phase III periodicity is severely altered in jejunal grafts. Homeostasis of enteric flora, however, is not influenced by the transplant procedure.
移行性肌电复合波（MMC）的第三相对于肠道菌群稳态的重要性已有充分记载。本研究的目的是在同基因大鼠模型中评估MMC变化对空肠移植物自我清除能力的影响。将Lewis大鼠整个空肠回肠近端的25%进行原位移植。然后将电极固定在移植物上。五只大鼠的天然肠段和五只具有类似空肠节段的大鼠作为对照。进行肌电记录直至第21天，此时处死动物以对进行肌电分析的节段及其相邻肠道进行细菌学分析。在所有记录过程中，所有动物均观察到MMC。由于第三相长期缺失与更频繁出现的第三相交替，移植组的第三相不规则。计算得出移植物第三相周期性的变异系数为48.74，天然肠段为14.79，节段性空肠为22.9。所有标本中发现的肠道菌群均由结肠样微生物组成。移植物中微生物的滴度与对照节段无差异。这些发现表明，空肠移植物中第三相周期性严重改变。然而，肠道菌群的稳态不受移植手术的影响。
