Pernthaler H, Pfurtscheller G, Klima G, Plattner R, Schmid T, Kofler M, Margreiter R
Department of Transplant Surgery, University Hospital, Innsbruck, Austria.
Eur Surg Res. 1993;25(5):316-20. doi: 10.1159/000129295.
In order to study the activity of noradrenergic nerve fibres along mesenteric arteries in small-intestinal grafts, the entire jejunoileum was transplanted heterotopically in an isogeneic rat model (group I, n = 12). To assess their influence on graft motility, 9 cm of jejunum were transplanted in an orthotopic position and three bipolar electrodes sutured to the seromuscular layer of the graft (group II, n = 10). Fasting motility was recorded up to postoperative day 42. Animals of group I were sacrificed from day 7 on at weekly intervals and mesenteries were analysed histochemically by fluorescence microscopy. After the 1st and 2nd week, grafts were found to be completely depleted of noradrenaline. At the end of the 3rd week, fluorescence became detectable along graft mesenteric arteries and showed normal intensity from the end of week 4. Migrating myoelectric complexes (MMCs) were present in all animals of group II. The variability of the MMC period (mean 12.6 min; SD 6.2 min) expressed as variation coefficient (median 36.5; 14.6-74) did not change during the observation period. From these findings it is concluded that there are no extrinsic noradrenergic activities in rat small-intestinal transplants for the first 3 postoperative weeks but they do recover there after. Their influence on graft function remains unclear: MMC periodicity, however, was no influenced.
为了研究小肠移植物中肠系膜动脉周围去甲肾上腺素能神经纤维的活性，在同基因大鼠模型中进行了全空肠回肠异位移植（第一组，n = 12）。为了评估它们对移植物运动的影响，将9 cm空肠移植到原位，并将三个双极电极缝合到移植物的浆肌层（第二组，n = 10）。记录术后42天内的禁食运动情况。第一组动物从第7天开始每周处死一次，通过荧光显微镜对肠系膜进行组织化学分析。在第1周和第2周后，发现移植物中的去甲肾上腺素完全耗尽。在第3周结束时，沿移植物肠系膜动脉可检测到荧光，从第4周结束时起显示正常强度。第二组所有动物均出现移行性肌电复合波（MMC）。以变异系数表示的MMC周期变异性（平均12.6分钟；标准差6.2分钟）（中位数36.5；14.6 - 74）在观察期内没有变化。从这些发现可以得出结论，大鼠小肠移植术后前3周不存在外在的去甲肾上腺素能活性，但之后会恢复。它们对移植物功能的影响仍不清楚：然而，MMC周期性未受影响。
