利用聚合酶链反应检测新生儿重症监护病房（NICU）新生儿尿液中的巨细胞病毒DNA。

Detection of cytomegalovirus DNA in urine from newborns in NICU using a polymerase chain reaction.

作者信息

Oda K, Oki S, Tsumura N, Nakao M, Motohiro T, Kato H

机构信息

Department of Pediatrics and Child Health, Kurume University School of Medicine, Japan.

出版信息

Kurume Med J. 1995;42(1):39-44. doi: 10.2739/kurumemedj.42.39.

DOI:10.2739/kurumemedj.42.39

PMID:7596090

Abstract

To investigate the natural course of viral shedding during the newborn period, the presence of Cytomegalovirus (CMV) DNA in specimens at 3, 7, 14, 21 and 28 days of life was examined using the polymerase chain reaction (PCR) method. At the 3rd day of life, the viral DNA positive rate in the urine was 7% (4/60), at the 7th day 7% (3/46), at the 14th day 10% (2/20), at the 21st day 10% (1/10), and at the 28th day 25% (1/5). CMV was also detected in samples co-cultivated with HeLa 229 cells and this positive rate was 5% (3/60). The viral positive rate in newborns did not correlate with the gestational age, body weight, or serum IgM level. Six congenital infection cases were identified; two of which were small-for-date babies (SFD) and three of which were born with premature rupture of membranes (PROM). They had no complications during the six months after birth.

摘要

为了研究新生儿期病毒脱落的自然病程，采用聚合酶链反应（PCR）方法检测了出生后3、7、14、21和28天标本中巨细胞病毒（CMV）DNA的存在情况。出生第3天，尿液中病毒DNA阳性率为7%（4/60），第7天为7%（3/46），第14天为10%（2/20），第21天为10%（1/10），第28天为25%（1/5）。在与HeLa 229细胞共培养的样本中也检测到了CMV，该阳性率为5%（3/60）。新生儿的病毒阳性率与胎龄、体重或血清IgM水平无关。确定了6例先天性感染病例；其中2例为小于胎龄儿（SFD），3例为胎膜早破（PROM）出生。他们在出生后的6个月内没有并发症。