血清中巨细胞病毒DNA的聚合酶链反应检测作为先天性巨细胞病毒感染的诊断试验
PCR detection of cytomegalovirus DNA in serum as a diagnostic test for congenital cytomegalovirus infection.
作者信息
Nelson C T, Istas A S, Wilkerson M K, Demmler G J
机构信息
Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA.
出版信息
J Clin Microbiol. 1995 Dec;33(12):3317-8. doi: 10.1128/jcm.33.12.3317-3318.1995.
Abstract
PCR detected cytomegalovirus (CMV) DNA in the serum of 18 of 18 infants with symptomatic congenital CMV infection, 1 of 2 infants with asymptomatic congenital CMV infection, and 0 of 32 controls. Serum CMV PCR provided a rapid, sensitive, and specific method for diagnosis of congenital CMV infection in infants who were symptomatic at birth.
摘要
聚合酶链反应（PCR）在18例有症状的先天性巨细胞病毒（CMV）感染婴儿的血清中检测到CMV DNA，在2例无症状先天性CMV感染婴儿中的1例检测到，而在32例对照中未检测到。血清CMV PCR为诊断出生时有症状的婴儿先天性CMV感染提供了一种快速、灵敏且特异的方法。
相似文献
1
PCR detection of cytomegalovirus DNA in serum as a diagnostic test for congenital cytomegalovirus infection.血清中巨细胞病毒DNA的聚合酶链反应检测作为先天性巨细胞病毒感染的诊断试验
J Clin Microbiol. 1995 Dec;33(12):3317-8. doi: 10.1128/jcm.33.12.3317-3318.1995.
2
PCR detection of cytomegalovirus DNA in serum as test for congenital cytomegalovirus infection.检测血清中巨细胞病毒DNA的聚合酶链反应作为先天性巨细胞病毒感染的检测方法。
J Clin Microbiol. 1996 Jul;34(7):1871-2. doi: 10.1128/JCM.34.7.1871-1872.1996.
3
Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection.干血斑中巨细胞病毒（CMV）DNA定量评估：CMV先天性感染的回顾性研究
J Clin Microbiol. 2007 Nov;45(11):3804-6. doi: 10.1128/JCM.01654-07. Epub 2007 Sep 26.
4
Diagnostic Value of Cytomegalovirus IgM Antibodies at Birth in PCR-Confirmed Congenital Cytomegalovirus Infection.出生时巨细胞病毒 IgM 抗体对 PCR 确诊先天性巨细胞病毒感染的诊断价值。
Int J Mol Sci. 2019 Jul 1;20(13):3239. doi: 10.3390/ijms20133239.
5
Evaluation of cytomegalovirus infections transmitted via breast milk in preterm infants with a real-time polymerase chain reaction assay.采用实时聚合酶链反应检测法评估经母乳传播的早产儿巨细胞病毒感染情况。
Pediatrics. 2003 Jun;111(6 Pt 1):1333-6. doi: 10.1542/peds.111.6.1333.
6
Prenatal diagnosis of congenital cytomegalovirus infection in 189 pregnancies with known outcome.189例已知妊娠结局的先天性巨细胞病毒感染的产前诊断
Prenat Diagn. 2001 May;21(5):362-77. doi: 10.1002/pd.59.
7
Dried blood spot real-time polymerase chain reaction assays to screen newborns for congenital cytomegalovirus infection.干血斑实时聚合酶链反应检测在新生儿先天性巨细胞病毒感染筛查中的应用。
JAMA. 2010 Apr 14;303(14):1375-82. doi: 10.1001/jama.2010.423.
8
Primary cytomegalovirus (CMV) infection in pregnancy: Diagnostic value of CMV PCR in saliva compared to urine at birth.孕妇原发性巨细胞病毒（CMV）感染：唾液 CMV-PCR 与出生时尿液相比的诊断价值。
J Clin Virol. 2019 Aug;117:33-36. doi: 10.1016/j.jcv.2019.05.015. Epub 2019 May 31.
9
Prevalence of congenital cytomegalovirus infection in symptomatic newborns under 3 weeks in Tehran, Iran.伊朗德黑兰3周龄以内有症状新生儿先天性巨细胞病毒感染的患病率
BMC Infect Dis. 2017 Oct 18;17(1):688. doi: 10.1186/s12879-017-2799-5.
引用本文的文献
1
Agreement Between Dried Blood Spot and Plasma PCR in Infants With Congenital Cytomegalovirus Infection.先天性巨细胞病毒感染婴儿干血斑与血浆聚合酶链反应的一致性
J Med Virol. 2025 Mar;97(3):e70257. doi: 10.1002/jmv.70257.
2
Congenital cytomegalovirus infection in newborns suspected of congenital rubella syndrome in Iran: a cross-sectional study.伊朗疑似先天性风疹综合征新生儿的先天性巨细胞病毒感染：一项横断面研究。
BMC Pediatr. 2024 Jan 10;24(1):31. doi: 10.1186/s12887-023-04502-3.
3
Prevalence and Characteristics of Cytomegalovirus Ocular Disease in Children: A Multi-Center Study.儿童巨细胞病毒眼病的患病率及特征：一项多中心研究。
Clin Ophthalmol. 2022 Jul 8;16:2209-2217. doi: 10.2147/OPTH.S364741. eCollection 2022.
4
Diagnostic Value of Cytomegalovirus IgM Antibodies at Birth in PCR-Confirmed Congenital Cytomegalovirus Infection.出生时巨细胞病毒 IgM 抗体对 PCR 确诊先天性巨细胞病毒感染的诊断价值。
Int J Mol Sci. 2019 Jul 1;20(13):3239. doi: 10.3390/ijms20133239.
5
Risk of congenital cytomegalovirus infection among HIV-exposed uninfected infants is not decreased by maternal nelfinavir use during pregnancy.孕期母亲使用奈非那韦并不能降低暴露于HIV但未受感染婴儿的先天性巨细胞病毒感染风险。
J Med Virol. 2016 Jun;88(6):1051-8. doi: 10.1002/jmv.24420. Epub 2015 Nov 18.
6
Comparison of automated nucleic acid extraction methods for the detection of cytomegalovirus DNA in fluids and tissues.比较用于检测体液和组织中巨细胞病毒 DNA 的自动化核酸提取方法。
PeerJ. 2014 Apr 8;2:e334. doi: 10.7717/peerj.334. eCollection 2014.
7
Diagnosis of congenital cytomegalovirus infection in high risk neonates.高危新生儿先天性巨细胞病毒感染的诊断。
Mediterr J Hematol Infect Dis. 2013 Jul 10;5(1):e2013049. doi: 10.4084/MJHID.2013.049. Print 2013.
8
Overview of the diagnosis of cytomegalovirus infection.巨细胞病毒感染的诊断概述。
Infect Disord Drug Targets. 2011 Oct;11(5):466-74. doi: 10.2174/187152611797636703.
9
Cytomegalovirus-specific, high-avidity IgG with neutralizing activity in maternal circulation enriched in the fetal bloodstream.母血循环中富含抗巨细胞病毒、高亲和力、具有中和活性的 IgG，可进入胎儿血流。
J Clin Virol. 2009 Dec;46 Suppl 4(Suppl 4):S58-63. doi: 10.1016/j.jcv.2009.10.004. Epub 2009 Oct 24.
10
Detection of HCMV DNA in placenta, amniotic fluid and fetuses of seropositive women by nested PCR.通过巢式聚合酶链反应检测血清反应阳性女性胎盘、羊水和胎儿中的人巨细胞病毒DNA
Eur J Pediatr. 2007 Jul;166(7):723-6. doi: 10.1007/s00431-006-0314-x. Epub 2006 Nov 3.
本文引用的文献
1
Early diagnosis of human cytomegalovirus disease in transplant recipients by DNA amplification in plasma.通过血浆中DNA扩增对移植受者人巨细胞病毒疾病进行早期诊断。
Transplantation. 1993 Aug;56(2):330-4. doi: 10.1097/00007890-199308000-00014.
2
Polymerase chain reaction to detect cytomegalovirus DNA in the cerebrospinal fluid of neonates with congenital infection.聚合酶链反应检测先天性感染新生儿脑脊液中的巨细胞病毒DNA。
J Infect Dis. 1994 Jun;169(6):1334-7. doi: 10.1093/infdis/169.6.1334.
3
Detection of cytomegalovirus DNA in sera of liver transplant recipients.肝移植受者血清中巨细胞病毒DNA的检测
J Clin Microbiol. 1994 Jun;32(6):1431-4. doi: 10.1128/jcm.32.6.1431-1434.1994.
4
Surveillance for congenital cytomegalovirus disease: a report from the National Congenital Cytomegalovirus Disease Registry.先天性巨细胞病毒疾病监测：来自国家先天性巨细胞病毒疾病登记处的报告。
Clin Infect Dis. 1995 Mar;20(3):665-70. doi: 10.1093/clinids/20.3.665.
5
Enzyme-linked immunosorbent assay for the detection of IgM-class antibodies to cytomegalovirus.用于检测巨细胞病毒IgM类抗体的酶联免疫吸附测定。
J Infect Dis. 1986 Jun;153(6):1152-5. doi: 10.1093/infdis/153.6.1152.
6
Immunoglobulin M antibodies detected by enzyme-linked immunosorbent assay and radioimmunoassay in the diagnosis of cytomegalovirus infections in pregnant women and newborn infants.通过酶联免疫吸附测定和放射免疫测定检测免疫球蛋白M抗体在孕妇和新生儿巨细胞病毒感染诊断中的应用。
J Clin Microbiol. 1985 Jun;21(6):930-5. doi: 10.1128/jcm.21.6.930-935.1985.
7
Epidemiologic characteristics of cytomegalovirus infection in mothers and their infants.
Am J Obstet Gynecol. 1988 May;158(5):1189-95. doi: 10.1016/0002-9378(88)90252-9.
8
A rapid method of sample preparation for detection of DNA viruses in human serum by polymerase chain reaction.一种通过聚合酶链反应检测人血清中DNA病毒的快速样本制备方法。
J Virol Methods. 1991 Nov;35(1):65-72. doi: 10.1016/0166-0934(91)90086-f.
9
Cytomegalovirus DNA in the sera of patients with cytomegalovirus pneumonia.
Br J Haematol. 1991 Oct;79(2):198-204. doi: 10.1111/j.1365-2141.1991.tb04522.x.
10
Improved amplification of cytomegalovirus DNA from urine after purification of DNA with glass beads.用玻璃珠纯化DNA后，尿中巨细胞病毒DNA的扩增得到改善。
Clin Chem. 1991 Nov;37(11):1945-9.
Zotero 插件