Heesbeen E C, Rijksen G, van Heugten H G, Verdonck L F
Department of Hematology, University Hospital Utrecht, The Netherlands.
Leuk Res. 1995 Jun;19(6):417-25. doi: 10.1016/0145-2126(95)00008-c.
The effect of serum on the antineoplastic action of the alkyl-lysophospholipid 1-octadecyl-2-O-methyl-sn-glycerol-3-phosphocholine (ET-18-OCH3) was studied in two human leukemia cell lines, HL60 and K562, and in leukemic cells of patients. Decreasing amounts of serum in the culture medium enhanced the cytotoxic action of ET-18-OCH3 dramatically in both cell lines and in the leukemic cells, as measured by cell survival and proliferation. Uptake of ET-18-OCH3 was likewise increased at reduced serum levels. Similar effects were obtained when fetal calf serum (FCS) in the culture medium was substituted by bovine serum albumin (BSA, fatty acid free). Selectivity of the alkyl-lysophospholipid at reduced serum or BSA level was demonstrated by clonogenic assays of normal marrow progenitor cells. Our study provides an optimalization of the purging conditions in autologous bone marrow transplantation, by using a low concentration of BSA during ET-18-OCH3 treatment (20 micrograms/ml for 4 h) in serum-free culture medium.
在两种人类白血病细胞系HL60和K562以及患者的白血病细胞中研究了血清对烷基溶血磷脂1-十八烷基-2-O-甲基-sn-甘油-3-磷酸胆碱（ET-18-OCH3）抗肿瘤作用的影响。通过细胞存活和增殖测定，培养基中血清量的减少显著增强了ET-18-OCH3在两种细胞系和白血病细胞中的细胞毒性作用。在血清水平降低时，ET-18-OCH3的摄取同样增加。当培养基中的胎牛血清（FCS）被无脂肪酸的牛血清白蛋白（BSA）替代时，也获得了类似的效果。通过对正常骨髓祖细胞的克隆形成试验证明了在血清或BSA水平降低时烷基溶血磷脂的选择性。我们的研究通过在无血清培养基中ET-18-OCH3处理期间（20微克/毫升，处理4小时）使用低浓度的BSA，为自体骨髓移植中的净化条件提供了优化。
