Trentin L, Pizzolo G, Zambello R, Agostini C, Morosato L, Sancetta R, Adami F, Vinante F, Chilosi M, Gallati H
Department of Clinical Medicine, Padua University School of Medicine, Italy.
Leukemia. 1995 Jun;9(6):1051-5.
The two tumor necrosis factor receptors (TNF-Rs) exist in their soluble form in different biological fluids. In this study we investigated the concentrations of soluble tumor necrosis factor receptors (sTNF-Rs) in the culture supernatants of leukemic cells and in the serum obtained from 33 patients: 12 with hairy cell leukemia (HCL) and 21 with B cell chronic lymphocytic leukemia (B-CLL). In seven patients with HCL, sTNF-Rs were also evaluated following in vivo treatment with interferon-alpha (IFN-alpha). Purified leukemic cells from patients with HCL and B-CLL spontaneously released sTNF-R75 but not sTNF-R55. The levels of sTNF-R75 were higher in supernatants obtained from cultured hairy cells than from cultures of B-CLL cells. The shedding of sTNF-R75 was further increased both in HCL and B-CLL subjects by some B cell-related stimuli, including BCGF, PMA, SAC and was partially inhibited by IFN-alpha in patients with HCL. Sera from HCL patients presented increased levels of both sTNF-Rs with respect to normal controls. Treatment of HCL patients with IFN-alpha resulted in a decrease in serum levels of sTNF-Rs, particularly sTNF-R75. These findings suggest that leukemic cells account for the increased serum levels of sTNF-R75 observed in patients with HCL and B-CLL, but not for sTNF-R55.
两种肿瘤坏死因子受体（TNF-Rs）以可溶性形式存在于不同生物体液中。在本研究中，我们检测了白血病细胞培养上清液以及33例患者血清中可溶性肿瘤坏死因子受体（sTNF-Rs）的浓度，其中12例为毛细胞白血病（HCL）患者，21例为B细胞慢性淋巴细胞白血病（B-CLL）患者。在7例HCL患者中，还在体内用α干扰素（IFN-α）治疗后评估了sTNF-Rs。来自HCL和B-CLL患者的纯化白血病细胞自发释放sTNF-R75，但不释放sTNF-R55。从培养的毛细胞获得的上清液中sTNF-R75水平高于B-CLL细胞培养物中的水平。包括BCGF、PMA、SAC在内的一些B细胞相关刺激进一步增加了HCL和B-CLL患者中sTNF-R75的释放，而IFN-α可部分抑制HCL患者中sTNF-R75的释放。与正常对照相比，HCL患者血清中两种sTNF-Rs水平均升高。用IFN-α治疗HCL患者导致血清中sTNF-Rs水平降低，尤其是sTNF-R75。这些发现表明，白血病细胞是HCL和B-CLL患者血清中sTNF-R75水平升高的原因，但不是sTNF-R55水平升高的原因。
