Reisner A, Olson J J, Yang J, Assietti R, Klemm J M, Girard P R
Department of Neurosurgery, Emory University, Atlanta, Georgia, USA.
Neurosurgery. 1995 Apr;36(4):806-12; discussion 813. doi: 10.1227/00006123-199504000-00023.
We report a simple explant technique to isolate and propagate endothelial cells from bovine cerebral arteries. The endothelial nature of the cells was confirmed by the presence of Factor VIII/von Willebrand antigen, the ability to phagocytize low-density lipoprotein, and the ability to be induced to express E-selectin. The lack of expression of the CD11c antigen and the absence of smooth muscle alpha-actin immunofluorescence suggested that the cultures were not contaminated with macrophages or smooth muscle cells, respectively. This technique yields pure cerebral arterial endothelial cell cultures, which will be of value for in vitro investigation of cerebrovascular physiology and disease processes.
我们报告了一种简单的外植体技术，用于从牛脑动脉中分离和培养内皮细胞。通过存在因子VIII/血管性血友病因子抗原、吞噬低密度脂蛋白的能力以及被诱导表达E-选择素的能力，证实了这些细胞的内皮特性。CD11c抗原的缺乏表达以及平滑肌α-肌动蛋白免疫荧光的缺失分别表明培养物未被巨噬细胞或平滑肌细胞污染。该技术可产生纯的脑动脉内皮细胞培养物，这对于脑血管生理学和疾病过程的体外研究具有重要价值。
