Morse L S, Sidikaro Y
Retina Research Laboratory, Jules Stein Eye Institute, UCLA School of Medicine 90024.
Curr Eye Res. 1990 Jul;9(7):631-42. doi: 10.3109/02713689008999578.
The invasion of Bruch's membrane and subsequently the subpigment epithelial and subretinal spaces by proliferating choroidal capillaries are prominent features of age-related macular degeneration. To facilitate study of this process, pure cell lines of bovine choroidal microvessel endothelium and pericytes were isolated and propagated in cell culture media. Their biological and biochemical properties are described. Choroidal microvascular endothelial cells displayed the characteristic cobblestone morphology, required fibronectin or gelatin for attachment to plastic culture dishes, displayed contact inhibition of growth and spontaneously formed tubules in culture. These cells contained antigens for Factor VIII and were able to phagocytize acetylated-low density lipoprotein. Choroidal microvascular pericytes were nonrectangular in shape and did not require substrate attachment factors. They were unable to form tubules in culture, were Factor VIII antigen negative, were unable to phagocytize acetylated-low density lipoprotein and did not display contact inhibition of growth. Choroidal microvascular pericytes contained antigens for muscle and nonmuscle actin.
脉络膜毛细血管增生侵入布鲁赫膜，随后侵犯色素上皮下和视网膜下间隙，是年龄相关性黄斑变性的显著特征。为便于研究这一过程，分离出牛脉络膜微血管内皮细胞和平滑肌细胞的纯细胞系，并在细胞培养基中进行增殖培养。文中描述了它们的生物学和生化特性。脉络膜微血管内皮细胞呈现出典型的鹅卵石样形态，附着于塑料培养皿需要纤连蛋白或明胶，具有生长接触抑制特性，且在培养中能自发形成小管。这些细胞含有因子VIII抗原，能够吞噬乙酰化低密度脂蛋白。脉络膜微血管平滑肌细胞形状不规则，不需要底物附着因子。它们在培养中不能形成小管，因子VIII抗原阴性，不能吞噬乙酰化低密度脂蛋白，也不具有生长接触抑制特性。脉络膜微血管平滑肌细胞含有肌肉和非肌肉肌动蛋白抗原。
