Zhou L, Hui F M, Morrison D A
Department of Biological Sciences, University of Illinois at Chicago, Illinois 60607-7020, USA.
Plasmid. 1995 Mar;33(2):127-38. doi: 10.1006/plas.1995.1014.
A new insertion sequence in Streptococcus pneumoniae was identified as a 1435-bp segment of the genome containing 24-bp terminal inverted repeats and flanked by 8-bp direct repeats. A copy of the element, named IS1167, adjacent to the comAB genes was sequenced; seven additional copies were found in the genome of strain CP1200 and relatives descended from strain R36A. Among 22 independent pneumococcal isolates, 11 contained copies of elements hybridizing to IS1167 in nine distinct restriction fragment patterns, with 3-12 copies each. The bulk of the element was occupied by two overlapping open reading frames, encoding basic proteins which together exhibited strong similarity to the full length of the putative transposase of the staphylococcal transposable element, IS1181, as well as significant similarity to those of seven additional known or putative insertion sequences related to the mycobacterial element, IS1096.
肺炎链球菌中的一个新插入序列被鉴定为基因组的一个1435 bp片段，其含有24 bp的末端反向重复序列，并两侧有8 bp的正向重复序列。对位于comAB基因附近的名为IS1167的元件拷贝进行了测序；在菌株CP1200及其源自菌株R36A的亲缘菌株的基因组中还发现了另外7个拷贝。在22株独立的肺炎球菌分离株中，11株含有与IS1167杂交的元件拷贝，呈现9种不同的限制性片段模式，每个分离株含有3 - 12个拷贝。该元件的大部分由两个重叠的开放阅读框占据，它们编码的碱性蛋白与葡萄球菌转座元件IS1181的推定转座酶全长具有高度相似性，并且与另外7个与分枝杆菌元件IS1096相关的已知或推定插入序列的相似性也很显著。
