Yao W, Yang Y, Chiao J
Shanghai Institute of Plant Physiology, Academia Sinica, Peoples Republic of China.
Plasmid. 1994 Nov;32(3):262-9. doi: 10.1006/plas.1994.1065.
An insertion sequence was found in Nocardia asteroides YP21. The element, designated IS204, is 1452 bp long and has 19/23 bp imperfect inverted repeats at its ends. The sequences of IS204 terminal inverted repeats have high homology with those of IS1096 from Mycobacterium smegmatis. An 8-bp duplication of target sequence was found at the insertion site. Sequence analysis revealed that IS204 contains an open reading frame of 1134 bp, which encodes a putative transposase similar to those found in IS1096 and in Tn4652 from Pseudomonas sp. EST1001. At least nine copies of IS204 are present in the genome of N asteroides YP21. The plasmid pCY 104::IS204 could be inserted with another copy of IS204 at a different site. The possible mechanisms are discussed.
在星形诺卡菌YP21中发现了一个插入序列。该元件命名为IS204，长度为1452 bp，两端有19/23 bp的不完全反向重复序列。IS204末端反向重复序列与耻垢分枝杆菌的IS1096序列具有高度同源性。在插入位点发现了一个8 bp的靶序列重复。序列分析表明，IS204包含一个1134 bp的开放阅读框，编码一种推定的转座酶，类似于在IS1096和假单胞菌属EST1001的Tn4652中发现的转座酶。在星形诺卡菌YP21的基因组中至少存在9个IS204拷贝。质粒pCY 104::IS204可以在另一个不同的位点插入另一个IS204拷贝。文中讨论了可能的机制。
