玫瑰毒鲉（Synanceia verrucosa Bloch和Schneider，1801）毒液的酶学特性及一种致死、降压和细胞溶解因子的纯化
Enzymatic properties of the stonefish (Synanceia verrucosa Bloch and Schneider, 1801) venom and purification of a lethal, hypotensive and cytolytic factor.
作者信息
Garnier P, Goudey-Perrière F, Breton P, Dewulf C, Petek F, Perrière C
机构信息
Laboratoire de Biologie et Contrôle des Organismes Parasites, Equipe Toxines et Insects, Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry, France.
出版信息
Toxicon. 1995 Feb;33(2):143-55. doi: 10.1016/0041-0101(94)00151-w.
The crude venom of the stonefish (Synanceia verrucosa) possesses numerous enzymatic properties (hyaluronidase, eight esterases and ten aminopeptidases). Verrucotoxin was isolated by DEAE and hydroxyapatite chromatography, followed by FPLC gel filtration on Superdex 200 HR 10/30. It was found to be a glycoprotein with a mol. wt of 322,000 +/- 2000, comprising four subunits, 2 alpha (83,000) and 2 beta (78,000). Verrucotoxin, as the crude venom, is lethal for mice, haemolyses washed rabbit erythrocytes and induces a fall in arterial pressure in anaesthetized rats.
玫瑰毒鲉（Synanceia verrucosa）的粗毒液具有多种酶活性（透明质酸酶、8种酯酶和10种氨肽酶）。通过DEAE和羟基磷灰石色谱法分离出疣状毒素，随后在Superdex 200 HR 10/30上进行快速蛋白质液相色谱凝胶过滤。发现它是一种糖蛋白，分子量为322,000±2000，由四个亚基组成，2个α亚基（83,000）和2个β亚基（78,000）。疣状毒素与粗毒液一样，对小鼠具有致死性，能使洗涤过的兔红细胞发生溶血，并导致麻醉大鼠的动脉血压下降。
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