从黑腹舞蛛毒液中分离出一种导致兔皮血管通透性增加的多肽。

Isolation of a polypeptide from Phoneutria nigriventer spider venom responsible for the increased vascular permeability in rabbit skin.

作者信息

Bento A C, Rego E, Pedroso-Mariani S R, Mancuso L C, Giglio J R, Novello J C, Marangoni S, Caracelli I, Oliveira B, Antunes E

机构信息

Department of Pharmacology, State University of Campinas (UNICAMP), SP, Brazil.

出版信息

Toxicon. 1995 Feb;33(2):171-8. doi: 10.1016/0041-0101(94)00148-2.

DOI:10.1016/0041-0101(94)00148-2

PMID:7597720

Abstract

Fractionation of Phoneutria nigriventer venom by Sephadex G-10 followed by ion-exchange chromatography yields a fraction (fraction XIII) which increases microvascular permeability in rabbit skin in vivo by activating the tissue kallikrein-kinin system. One polypeptide (PNV3) with the ability to increase microvascular permeability in the rabbit skin in vivo was isolated from fraction XIII and biochemically characterized. PNV3 has 132 amino acid residues with a calculated mol. wt of 14,475. This polypeptide showed the following N-terminal sequence: AVFAIQDQPC. Amino acid analysis indicated the presence of six disulfide bridges and a high content of Glx (20%). Pairwise comparison of PNV3 amino acid sequence with 27 other spider venom polypeptides and proteins indicated that PNV3 presents high similarity (60-70%) with other toxins (Tx2.1, Tx2.5 and Tx2.6) isolated from P. nigriventer venom.

摘要

用葡聚糖凝胶G - 10对黑腹遁蛛毒液进行分级分离，随后进行离子交换色谱法，得到一个组分（组分XIII），该组分通过激活组织激肽释放酶 - 激肽系统，在体内增加兔皮肤的微血管通透性。从组分XIII中分离出一种具有在体内增加兔皮肤微血管通透性能力的多肽（PNV3），并对其进行了生化特性鉴定。PNV3有132个氨基酸残基，计算分子量为14,475。该多肽显示出以下N端序列：AVFAIQDQPC。氨基酸分析表明存在六个二硫键，且谷氨酸（20%）含量较高。将PNV3氨基酸序列与其他27种蜘蛛毒液多肽和蛋白质进行成对比较表明，PNV3与从黑腹遁蛛毒液中分离出的其他毒素（Tx2.1、Tx2.5和Tx2.6）具有高度相似性（60 - 70%）。