从“武装”蜘蛛黑腹捕鸟蛛毒液中分离神经毒性肽。

Isolation of neurotoxic peptides from the venom of the 'armed' spider Phoneutria nigriventer.

作者信息

Rezende Júnior L, Cordeiro M N, Oliveira E B, Diniz C R

机构信息

Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

出版信息

Toxicon. 1991;29(10):1225-33. doi: 10.1016/0041-0101(91)90195-w.

DOI:10.1016/0041-0101(91)90195-w

PMID:1801316

Abstract

Three neurotoxic fractions, lethal to mice, were isolated from the venom of the spider Phoneutria nigriventer, by gel filtration and reverse phase chromatography (Phoneutria toxins 1, 2 and 3). These toxins have mol. wts in the range 6000-9000, and have different amino acid compositions and N-terminal amino acid sequences. The toxins also differ in the lethality and signs they cause in mice after intracerebro-ventricular injection. The median LD50 being respectively for the whole venom, toxins 1, 2 and 3, 47 +/- 5 micrograms, 45 +/- 4 micrograms, 1.7 +/- 0.7 micrograms and 137 +/- 10 micrograms/kg mouse. Toxins 1 and 2 induce excitatory symptoms in mice and toxin 3 a flaccid paralysis with an ED50 of 40 +/- 5 micrograms/kg mouse as measured also by intracerebro-ventricular injection. The presence in the venom of a non-neurotoxic, smooth muscle active peptide is also described.

摘要

通过凝胶过滤和反相色谱法，从黑腹捕鸟蛛的毒液中分离出三种对小鼠具有神经毒性的组分（捕鸟蛛毒素1、2和3）。这些毒素的分子量在6000 - 9000范围内，具有不同的氨基酸组成和N端氨基酸序列。这些毒素在脑室注射后对小鼠的致死率和所引发的症状也有所不同。全毒液、毒素1、2和3的半数致死量（LD50）分别为47±5微克、45±4微克、1.7±0.7微克和137±10微克/千克小鼠。毒素1和2会在小鼠中引发兴奋症状，毒素3则会导致弛缓性麻痹，通过脑室注射测量其半数有效量（ED50）为40±5微克/千克小鼠。文中还描述了毒液中存在一种非神经毒性的、对平滑肌有活性的肽。