Nioka S, Miwa M, Orel S, Shnall M, Haida M, Zhao S, Chance B
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104, USA.
Adv Exp Med Biol. 1994;361:171-9. doi: 10.1007/978-1-4615-1875-4_22.
Since an increasing number of breast cancers have been reported in recent years, there is a need for improving techniques for early detection of the breast cancer. Here we tested a time gated optical imaging technique as a tool for imaging human breast. Pulsed laser light at wavelengths of 780 and 830 nm are transmitted through human breast tissues and time spectra of the diffused light through the tissue are recorded over nanoseconds. Data from different locations are acquired and used to reconstruct a two dimensional image as a set of spectra in pixel form. The imaging consists of absorption and scattering coefficients, and the absorption coefficients at the two wavelengths are related to oxygen concentration and blood volume. The analysis of these coefficients is based upon the early arrival photons, therefore allowing construction of a better image than those from the current diaphanography. We demonstrate images of breast cancer, cysts created after lumpectomy, and consequences of radiation therapy. Results show that time gated optical imaging can image oxygen concentration in the cancerous and fibrotic breasts. Resolution of the imaging for smaller tumor size needs to be improved.
近年来，乳腺癌的报告病例数不断增加，因此需要改进乳腺癌的早期检测技术。在此，我们测试了一种时间选通光学成像技术，作为一种用于人体乳房成像的工具。波长为780和830 nm的脉冲激光穿过人体乳房组织，并在纳秒级记录透过组织的漫射光的时间光谱。采集来自不同位置的数据，并用于重建二维图像，该图像以像素形式呈现为一组光谱。成像包括吸收系数和散射系数，这两个波长处的吸收系数与氧浓度和血容量有关。对这些系数的分析基于早期到达的光子，因此能够构建出比当前透照法更好的图像。我们展示了乳腺癌、肿块切除术后形成的囊肿以及放射治疗结果的图像。结果表明，时间选通光学成像可以对癌性和纤维化乳房中的氧浓度进行成像。对于较小肿瘤大小的成像分辨率有待提高。
