Regoli M, Borghesi C, Bertelli E, Comparini L
Institute of Human Anatomy, University of Siena, Italy.
Ann Anat. 1995 May;177(3):229-35. doi: 10.1016/S0940-9602(11)80191-3.
The lymphatic network in Peyer's patches has been previously studied by scanning electron microscopy in rabbit and sheep. So far, data on the Peyer's patch lymphatic network obtained by light and transmission electron microscopy are still lacking. The present work was carried out on several series of consecutive thick and semithin sections of mouse Peyer's patches. Ultrastructural analyses were performed on ultrathin sections by traditional transmission electron microscopy. Lymph vessels were detected in the parafollicular and subfollicular areas of Peyer's patches. Two independent lymphatic plexuses, the muscularis mucosae lymphatic plexus and the subfollicular plexus, were identified respectively in the mucosal and submucosal layers. These lymphatic plexuses joined outside the patch, both draining into the submucosal lymphatic network of the small intestine. At the ultrastructural level, the muscularis mucosae lymphatic plexus, and the lymph vessels draining into it, showed a close association with bundles of smooth muscle cells. Numerous nerve fibres were detected in proximity to the lymphatic endothelium and, in some cases, synapse-like neuroendothelial associations were observed. We report here the lymph vessel organization observed in mouse Peyer's patches and discuss the meaning of the presence of subendothelial nerve terminals.
此前已通过扫描电子显微镜对兔和绵羊的派尔集合淋巴结中的淋巴网络进行了研究。到目前为止，通过光学显微镜和透射电子显微镜获得的关于派尔集合淋巴结淋巴网络的数据仍然缺乏。本研究对小鼠派尔集合淋巴结的几个连续厚切片和半薄切片系列进行。通过传统透射电子显微镜对超薄切片进行超微结构分析。在派尔集合淋巴结的滤泡旁和滤泡下区域检测到淋巴管。分别在黏膜层和黏膜下层鉴定出两个独立的淋巴丛，即黏膜肌层淋巴丛和滤泡下丛。这些淋巴丛在淋巴结外相连，均汇入小肠的黏膜下淋巴网络。在超微结构水平上，黏膜肌层淋巴丛及其汇入的淋巴管与平滑肌细胞束密切相关。在淋巴管内皮附近检测到大量神经纤维，在某些情况下，观察到类似突触的神经内皮关联。我们在此报告在小鼠派尔集合淋巴结中观察到的淋巴管组织，并讨论内皮下单神经末梢存在的意义。
