Ohtani O, Kikuta A, Ohtsuka A, Murakami T
Department of Anatomy, Okayama University School of Medicine, Japan.
Anat Rec. 1991 Feb;229(2):251-8. doi: 10.1002/ar.1092290212.
The organization of the network of collagen fibers of rabbit Peyer's patches was examined by scanning electron microscopy (SEM) in alkali-water macerated tissues. The relationship between this network and the reticular cells within it was further studied by SEM of ultrasonicated tissues. Collagen fibrils (about 60 nm in diameter) formed collagen fibers or sheets. There were sheets of collagen fibrils with numerous pores beneath the patch dome epithelium. Within the patches, collagen fibers repeatedly divided and fused, forming the reticular network. The reticular network within the follicle was looser than within the dome, the corona, or the interfollicular area. The latter three compartments showed similar structures and consisted of numerous intercommunicating small subcompartments. Reticular cells were in contact with groups of free cells lodged in these subcompartments within the reticular network. Reticular cell processes with numerous fenestrations embraced not only collage fibers forming the reticular network, but also sheaths of collagen fibers of blood and lymphatic vessels. Sheaths of collagen fibers of high endothelial venules and lymphatic vessels were also fenestrated, indicating the sites through which lymphocytes and other free cells migrate. These results indicate that the reticular network of Peyer's patches is organized so as to facilitate migration and lodging of free cells and thus facilitate antigen-to-cell and cell-to-cell interactions during an immune response. The naked areas on the collagen fibers seem to provide a scaffolding for free cells during their migration.
通过扫描电子显微镜（SEM）对碱水浸软组织中的兔派尔集合淋巴结胶原纤维网络结构进行了研究。通过对超声处理后的组织进行SEM进一步研究了该网络与其内部网状细胞之间的关系。胶原原纤维（直径约60nm）形成胶原纤维或薄片。在集合淋巴结圆顶上皮下方有带有许多孔隙的胶原原纤维薄片。在集合淋巴结内，胶原纤维反复分支并融合，形成网状网络。滤泡内的网状网络比圆顶、冠部或滤泡间区域内的更松散。后三个区域显示出相似的结构，由许多相互连通的小隔室组成。网状细胞与位于网状网络内这些隔室中的游离细胞群接触。具有许多窗孔的网状细胞突起不仅包绕形成网状网络的胶原纤维群，还包绕血管和淋巴管的胶原纤维鞘。高内皮微静脉和淋巴管的胶原纤维鞘也有窗孔，表明淋巴细胞和其他游离细胞迁移的部位。这些结果表明，派尔集合淋巴结的网状网络的组织方式有利于游离细胞的迁移和驻留，从而在免疫反应期间促进抗原与细胞以及细胞与细胞之间的相互作用。胶原纤维上的裸露区域似乎在游离细胞迁移过程中为其提供了一个支架。
