Chandra P, Ebener U, Steel L K, Laube H, Kornhuber B, Thorbeck R R
Klin Padiatr. 1978 Jan;190(1):9-27.
This article concerns the molecular mechanisms by which RNA tumor viruses, commonly called as oncornaviruses, transfer their genetic information from the genomic RNA (70 s RNA) of the virions to the cellular DNA, leading to neoplastic transformations. The article describes biochemical and serological properties of reverse transcriptase, its role in the life cycle of RNA tumor viruses and broader implications to molecular biology. In this connection, the authors report their own findings on the role of reverse transcriptase in a preleukemic disease, myelofibrosis. This enzyme, discovered in their laboratory, is antigenically closely related to reverse transcriptase of certain primate RNA tumor viruses, and of human leukemic cells. The article also describes the role of reverse transcriptase inhibitors in viral oncogenesis. Of particular interest, is the partially thiolated polycytidylic acid (MPC) which has been developed by the authors, and is known to have a very high binding affinity to the viral reverse transcriptase. The implication of these basic data on the clinical effectivity of MPC in human leukemia, documented in a few cases, has been discussed.
本文关注RNA肿瘤病毒（通常称为致癌RNA病毒）将其遗传信息从病毒粒子的基因组RNA（70s RNA）转移至细胞DNA从而导致肿瘤转化的分子机制。文章描述了逆转录酶的生化和血清学特性、其在RNA肿瘤病毒生命周期中的作用以及对分子生物学更广泛的影响。就此，作者报告了他们自己关于逆转录酶在白血病前期疾病骨髓纤维化中作用的研究发现。这种在他们实验室中发现的酶，在抗原性上与某些灵长类RNA肿瘤病毒及人类白血病细胞的逆转录酶密切相关。文章还描述了逆转录酶抑制剂在病毒致癌过程中的作用。特别值得关注的是作者研发的部分硫醇化聚胞苷酸（MPC），已知其对病毒逆转录酶具有非常高的结合亲和力。文中讨论了这些基础数据对MPC在少数已记录病例中的人类白血病临床疗效的影响。
