Lobmaier C, Schalkhammer T, Hawa G, Ecker B, Pittner F
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Luswig Boltzmann Forschungsstelle für Biochemie, Austria.
J Mol Recognit. 1995 Jan-Apr;8(1-2):146-50. doi: 10.1002/jmr.300080125.
This article details a new type of biosensor for the simultaneous analysis of glucose, glutamate and glutamine in complex biological fluids like fermentation broths and blood. Simultaneous analysis was made possible by the application of different enzyme layers onto different electrodes of one photostructurized sensor. Photostructuring was done by means of a new developed photopolymer. Preparation of the photopolymer and the enzyme layers as well as the characterization of the sensors thus constructed with respect to linearity, response time and sensitivity are described.
本文详细介绍了一种新型生物传感器,用于同时分析发酵液和血液等复杂生物流体中的葡萄糖、谷氨酸和谷氨酰胺。通过在一个光结构化传感器的不同电极上应用不同的酶层,实现了同时分析。光结构化是通过一种新开发的光聚合物来完成的。描述了光聚合物和酶层的制备,以及由此构建的传感器在线性、响应时间和灵敏度方面的特性。