Sungur Sibel, Emregül Emel, Günendi Gülendem, Numanoğlu Yasemin
Faculty of Science, Department of Chemistry, Biochemistry, Ankara University, 06100 Beşelver, Ankara, Turkey.
J Biomater Appl. 2004 Apr;18(4):265-77. doi: 10.1177/0885328204040950.
In this article we describe the preparation of a new glucose biosensor based on electrode coating with glucose oxidase-immobilized gelatin. Glucose oxidase was immobilized onto gelatin by cross-linking with chromium III acetate. Properties of the immobilized glucose oxidase enzyme electrode have been studied and results were compared with free enzyme electrode. Effect of temperature and pH variations, kinetic properties, and re-usability were among the subjects analyzed. Also interfering effect of the electro-active compounds was investigated and their influence on the behavior of glucose oxidase electrode was determined. The response time of the developed glucose oxidase enzyme electrode was 60 s with a detection limit of 0.25 mM and preserved its accuracy within the 2 months period of reuse experiments.
在本文中，我们描述了一种基于用固定有葡萄糖氧化酶的明胶包覆电极制备新型葡萄糖生物传感器的方法。葡萄糖氧化酶通过与醋酸铬（III）交联固定在明胶上。对固定化葡萄糖氧化酶酶电极的性能进行了研究，并将结果与游离酶电极进行了比较。分析的主题包括温度和pH变化的影响、动力学性质以及可重复使用性。此外，还研究了电活性化合物的干扰作用，并确定了它们对葡萄糖氧化酶电极行为的影响。所开发的葡萄糖氧化酶酶电极的响应时间为60秒，检测限为0.25 mM，并且在重复使用实验的2个月期间内保持了其准确性。
