Radda G K, Odoom J, Kemp G, Taylor D J, Thompson C, Styles P
MRC Biochemical and Clinical Magnetic Resonance Unit, Oxford Radcliffe Hospital, UK.
Biochim Biophys Acta. 1995 May 24;1271(1):15-9. doi: 10.1016/0925-4439(95)00004-n.
Mitochondrial function in muscle in vivo can be quantitatively evaluated using 31-phosphorus nuclear magnetic resonance. In resting muscle, the concentrations of ions (e.g. H+, Na+) and two of the major bioenergetic components (inorganic phosphate and creatine) are determined by regulated transcellular transport processes. During recovery after exercise the kinetics and control of mitochondrial ATP synthesis can be established. During exercise the relative contributions to ATP synthesis of phosphocreatine (using creatine kinase), anaerobic glycogenolysis and oxidative phosphorylation are dissected and have been shown to change with time. The consequences of mitochondrial lesions and dysfunctions on these processes have been summarised.
利用31-磷核磁共振可对体内肌肉的线粒体功能进行定量评估。在静息肌肉中，离子（如H⁺、Na⁺）以及两种主要生物能量成分（无机磷酸盐和肌酸）的浓度由调节性跨细胞转运过程决定。运动后的恢复过程中，可确定线粒体ATP合成的动力学和调控机制。运动期间，可剖析磷酸肌酸（利用肌酸激酶）、无氧糖酵解和氧化磷酸化对ATP合成的相对贡献，并且这些贡献已被证明会随时间变化。线粒体损伤和功能障碍对这些过程的影响已被总结。
