Kobayashi Y, Uoshima N, Kimura S, Tanaka K, Wada K, Ozawa M, Maruo N, Kondo M
1st Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Int J Hematol. 1995 Apr;61(3):117-22. doi: 10.1016/0925-5710(95)00358-y.
To clarify the biological behavior of micromegakaryocytes in myelodysplastic syndrome (MDS), the relationship between the morphological classification and the ploidy of megakaryocytes was studied in bone marrow aspirates obtained from patients with MDS and from normal controls. The morphology was determined according to Feinendegen's classification, which is considered to reflect megakaryocytic maturation, and the ploidy was determined by microcytofluorometry, using 4',6-diamidino-2-phenylindole (DAPI) staining after the removal of Wright-Giemsa stain. Most micromegakaryocytes (i.e., megakaryocytes < 20 microns in diameter) in MDS were morphologically mature, as were those in the normal controls. The peak micromegakaryocytic ploidy was 4N or 8N, whereas that of the megakaryocytes in normal controls was 16N. These findings indicated that the micromegakaryocytes in MDS were morphologically mature but had impaired polyploidization.
为阐明骨髓增生异常综合征（MDS）中微巨核细胞的生物学行为，对MDS患者及正常对照者骨髓穿刺物中巨核细胞的形态学分类与倍性之间的关系进行了研究。形态学依据Feinendegen分类法确定，该分类法被认为可反映巨核细胞成熟情况，倍性则通过微量细胞荧光测定法确定，在去除瑞氏-吉姆萨染色后，使用4',6-二脒基-2-苯基吲哚（DAPI）染色。MDS中的大多数微巨核细胞（即直径<20微米的巨核细胞）在形态上是成熟的，正常对照中的微巨核细胞也是如此。微巨核细胞的倍性峰值为4N或8N，而正常对照中巨核细胞的倍性峰值为16N。这些发现表明，MDS中的微巨核细胞在形态上是成熟的，但多倍体化受损。
