Kawaguchi M, Nehashi Y, Aizawa S, Toyama K
First department of Internal Medicine, Tokyo Medical College, Japan.
Eur J Haematol. 1990 Feb;44(2):89-94. doi: 10.1111/j.1600-0609.1990.tb00356.x.
23 patients with myelodysplastic syndromes (MDS) and 8 normal controls were analyzed for dysmegakaryopoiesis (DMP) in the bone marrow by alkaline phosphatase anti-alkaline phosphatase (APAAP) technique and by conventional May-Giemsa staining. In the immunocytochemical study, monoclonal antibody (MoAb) against glycoprotein (GP) IIb/IIIa was utilized to demonstrate megakaryocytic cells. 91% (21/23) of MDS cases were detected as having DMP by APAAP method, while only 52% (12/23) were detectable by Giemsa stain. There were difficulties in recognizing small micromegakaryocytes (micro MKs), designated as type 1 atypical MKs, by Giemsa staining. Furthermore, megakaryoblasts (MKBs) were detectable only by APAAP technique. In 8 normal controls, no type 1 and type 3 atypical MKs (round shaped multinuclear MKs) were observed either by Giemsa staining or by the APAAP method, suggesting that they are a distinctive feature of MDS. These results indicate the necessity of immunocytochemical technique for accurate recognition of DMP in MDS.
采用碱性磷酸酶抗碱性磷酸酶（APAAP）技术和传统的美吉姆萨染色法，对23例骨髓增生异常综合征（MDS）患者和8名正常对照者的骨髓巨核细胞生成异常（DMP）情况进行了分析。在免疫细胞化学研究中，使用抗糖蛋白（GP）IIb/IIIa单克隆抗体（MoAb）来识别巨核细胞。通过APAAP法检测出91%（21/23）的MDS病例存在DMP，而吉姆萨染色法仅能检测出52%（12/23）的病例。吉姆萨染色法在识别被指定为1型非典型巨核细胞的小微型巨核细胞（微型MKs）时存在困难。此外，仅通过APAAP技术才能检测到原巨核细胞（MKBs）。在8名正常对照者中，吉姆萨染色法和APAAP法均未观察到1型和3型非典型巨核细胞（圆形多核巨核细胞），这表明它们是MDS的一个显著特征。这些结果表明，免疫细胞化学技术对于准确识别MDS中的DMP是必要的。
