Jouk P S, Usson Y, Michalowicz G, Parazza F
Equipe de Reconnaissance des Formes et de Microscopie Quantitative, CERMO, Centre Hospitalier Universitaire de Grenoble, France.
Microsc Res Tech. 1995 Apr 15;30(6):480-90. doi: 10.1002/jemt.1070300605.
The study of the topological organisation of myocardial cells is a basic requirement for the understanding of the mechanical design of the normal and pathological heart. We developed a technique based on multiparametric image analysis of transmitted polarized light to generate maps of the azimuth and the elevation angles of the myocardial cells. The properties of birefringence of the myocardium embedded in methylmetacrylate were measured in papillary muscles with monitored 3D orientation. This birefringence is positive uniaxial with a 0 degree extinction angle when the axis of the fiber is parallel to the axis of the polarizer or the analyzer. Thick sections were studied between crossed polars, and four images of each section were digitized for an angle of the polarizer with the section varying from 0-67.5 degrees in steps of 22.5 degrees. The amounts of transmitted light for each setup of the polarizer were combined in order to extract the values of the azimuth angle (modulo 90 degrees) and the elevation angle of the myocardial cells, according to the Johannsen equation. The respective maps of these angles were calculated and then assessed with confocal scanning laser microscopy. This method provides an efficient and accurate tool for the study of the histological architecture of the fetal and neonatal heart.
心肌细胞拓扑组织的研究是理解正常和病理心脏机械设计的基本要求。我们开发了一种基于透射偏振光多参数图像分析的技术，以生成心肌细胞方位角和仰角图。在监测三维取向的乳头肌中测量嵌入甲基丙烯酸甲酯的心肌的双折射特性。当纤维轴与偏振器或检偏器的轴平行时，这种双折射是正单轴的，消光角为0度。在正交偏振器之间研究厚切片，对于偏振器与切片的角度以22.5度的步长从0 - 67.5度变化的情况，每个切片数字化四张图像。根据约翰森方程，将偏振器每种设置下的透射光量进行组合，以提取心肌细胞的方位角（模90度）和仰角值。计算这些角度的各自图谱，然后用共聚焦扫描激光显微镜进行评估。该方法为研究胎儿和新生儿心脏的组织学结构提供了一种高效且准确的工具。
