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在新建立的人横纹肌肉瘤细胞系中的体外分化与增殖

In vitro differentiation and proliferation in a newly established human rhabdomyosarcoma cell line.

作者信息

Ogose A, Motoyama T, Hotta T, Watanabe H

机构信息

Department of Pathology, Niigata University School of Medicine, Japan.

出版信息

Virchows Arch. 1995;426(4):385-91. doi: 10.1007/BF00191348.

DOI:10.1007/BF00191348
PMID:7599791
Abstract

A newly established cell line, designated NRS-1, was derived from an alveolar rhabdomyosarcoma that developed in the left forearm of a 7-year-old girl. The cell line had a t(2; 13) chromosomal translocation. Reverse transcription-polymerase chain reaction demonstrated that 5' PAX3-3' FKHR chimeric transcript was expressed in NRS-1 cells. NRS-1 cells showed myogenic differentiation without any particular stimulus in vitro and exhibited various kinds of muscle markers. All-trans retinoic acid promoted cell differentiation in the myogenic direction. Transforming growth factor-beta (TGF-beta) inhibited myogenic differentiation of those cells and promoted cell proliferation.

摘要

一种新建立的细胞系，命名为NRS-1，源自一名7岁女孩左前臂发生的肺泡横纹肌肉瘤。该细胞系存在t(2; 13)染色体易位。逆转录-聚合酶链反应表明，5' PAX3-3' FKHR嵌合转录本在NRS-1细胞中表达。NRS-1细胞在体外无需任何特殊刺激即可进行肌源性分化，并表现出各种肌肉标志物。全反式维甲酸促进细胞向肌源性方向分化。转化生长因子-β（TGF-β）抑制这些细胞的肌源性分化并促进细胞增殖。

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In vitro differentiation and proliferation in a newly established human rhabdomyosarcoma cell line.在新建立的人横纹肌肉瘤细胞系中的体外分化与增殖
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2
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Cancer Res. 1991 Sep 15;51(18):4882-7.
4
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