在果蝇发育过程中，丝切蛋白突变会破坏多个肌动蛋白依赖性过程。
Profilin mutations disrupt multiple actin-dependent processes during Drosophila development.
作者信息
Verheyen E M, Cooley L
机构信息
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
出版信息
Development. 1994 Apr;120(4):717-28. doi: 10.1242/dev.120.4.717.
The chickadee gene of Drosophila encodes profilin, a small actin binding protein. We present the first analysis of the effects of profilin deletion in a multicellular organism. Genomic deletions of the chickadee locus result in a late embryonic lethal phenotype indicating that profilin is essential in flies. In addition, viable alleles of chickadee with defects in oogenesis, spermatogenesis and bristle formation provide insight into profilin function in a variety of cell types. Defects in oogenesis include the previously described failure to assemble nurse cell actin filament bundles in addition to abnormal regulation of mitosis, binucleate cells and stalled cell migration. Malformed bristles are a result of aberrant actin assembly. Monoclonal antibodies against Drosophila profilin were generated to study profilin's cellular and subcellular localization.
果蝇的山雀基因编码一种小的肌动蛋白结合蛋白——肌动蛋白单体结合蛋白。我们首次分析了肌动蛋白单体结合蛋白缺失在多细胞生物中的影响。山雀基因座的基因组缺失导致胚胎后期致死表型，这表明肌动蛋白单体结合蛋白在果蝇中是必不可少的。此外，山雀基因的可行等位基因在卵子发生、精子发生和刚毛形成方面存在缺陷，这为了解肌动蛋白单体结合蛋白在多种细胞类型中的功能提供了线索。卵子发生缺陷包括除了有丝分裂、双核细胞和细胞迁移停滞的异常调节外，还包括先前描述的无法组装滋养细胞肌动蛋白丝束。畸形刚毛是肌动蛋白组装异常的结果。我们制备了针对果蝇肌动蛋白单体结合蛋白的单克隆抗体，以研究肌动蛋白单体结合蛋白的细胞和亚细胞定位。
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