Eldon E, Kooyer S, D'Evelyn D, Duman M, Lawinger P, Botas J, Bellen H
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA.
Development. 1994 Apr;120(4):885-99. doi: 10.1242/dev.120.4.885.
We have isolated and characterized a novel gene, named 18 wheeler (18w) for its unique segmental expression pattern in Drosophila embryos and expression in cells that migrate extensively. 18 wheeler transcripts accumulate in embryos in a pattern reminiscent of segment polarity genes. Mutations in 18w cause death during larval development and early adulthood. Escaping mutant adults often display leg, antenna, and wing deformities, presumably resulting from improper eversion of imaginal discs. Sequence analysis indicates that 18w encodes a transmembrane protein with an extracellular moiety containing many leucine rich repeats and cysteine motifs, and an intracellular domain bearing homology to the cytoplasmic portion of the interleukin-1-receptor. Expression of 18W protein in non-adhesive Schneider 2 cells promotes rapid and robust aggregation of cells. Analysis of the expression of 18w in different mutant backgrounds shows that it is under control of segment polarity and homeotic genes. The data suggest that the 18W protein participates in the developmental program specified by segmentation and homeotic genes as a cell adhesion or receptor molecule that facilitates cell movements.
我们分离并鉴定了一个新基因，因其在果蝇胚胎中独特的分段表达模式以及在广泛迁移的细胞中的表达，将其命名为18轮车（18w）。18轮车转录本在胚胎中积累的模式让人联想到分段极性基因。18w突变会导致幼虫发育和成年早期死亡。存活下来的突变成虫常常表现出腿部、触角和翅膀畸形，推测这是由于成虫盘外翻不当所致。序列分析表明，18w编码一种跨膜蛋白，其细胞外部分含有许多富含亮氨酸的重复序列和半胱氨酸基序，细胞内结构域与白细胞介素-1受体的胞质部分具有同源性。18W蛋白在非黏附性的施奈德2细胞中的表达促进了细胞的快速且强烈聚集。对18w在不同突变背景下的表达分析表明，它受分段极性基因和同源异型基因的调控。数据表明，18W蛋白作为一种促进细胞运动的细胞黏附或受体分子，参与了由分段基因和同源异型基因指定的发育程序。
