果蝇的融合体是一种生殖系特异性细胞器，含有膜骨架蛋白，并在囊肿形成中发挥作用。

The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation.

作者信息

Lin H, Yue L, Spradling A C

机构信息

Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA.

出版信息

Development. 1994 Apr;120(4):947-56. doi: 10.1242/dev.120.4.947.

DOI:10.1242/dev.120.4.947

PMID:7600970

Abstract

Oogenesis in Drosophila takes place within germline cysts that support polarized transport through ring canals interconnecting their 15 nurse cells and single oocyte. Developing cystocytes are spanned by a large cytoplasmic structure known as the fusome that has been postulated to help form ring canals and determine the pattern of nurse cell-oocyte interconnections. We identified the adducin-like hts product and alpha-spectrin as molecular components of fusomes, discovered a related structure in germline stem cells and documented regular associations between fusomes and cystocyte centrosomes. hts mutations completely eliminated fusomes, causing abnormal cysts containing a reduced number of cells to form. Our results imply that Drosophila fusomes are required for ovarian cyst formation and suggest that membrane skeletal proteins regulate cystocyte divisions.

摘要

果蝇的卵子发生在生殖系囊肿内，这些囊肿通过连接其15个滋养细胞和单个卵母细胞的环管支持极化运输。发育中的囊肿细胞被一种称为融合体的大型细胞质结构所贯穿，据推测该结构有助于形成环管并确定滋养细胞与卵母细胞的连接模式。我们鉴定出类内收蛋白hts产物和α-血影蛋白是融合体的分子成分，在生殖系干细胞中发现了一种相关结构，并记录了融合体与囊肿细胞中心体之间的规律关联。hts突变完全消除了融合体，导致形成细胞数量减少的异常囊肿。我们的结果表明果蝇的融合体是卵巢囊肿形成所必需的，并提示膜骨架蛋白调节囊肿细胞的分裂。