Barth K A, Wilson S W
Developmental Biology Research Centre, Randall Institute, King's College London, UK.
Development. 1995 Jun;121(6):1755-68. doi: 10.1242/dev.121.6.1755.
We have isolated zebrafish nk2.2, a member of the Nk-2 family of homeobox genes. nk2.2 is expressed in a continuous narrow band of cells along a boundary zone demarcating the location at which two of the earliest nuclei in the brain differentiate. This band of cells is located within a few cell diameters of cells expressing the signalling molecule sonic hedgehog/vertebrate hedgehog-1 (shh/vhh-1). Injection of shh/vhh-1 RNA results in ectopic expression of nk2.2 and concomitant abnormalities in the forebrain and eyes. Moreover, cyclops mutant embryos, which initially lack neurectodermal expression of shh/vhh-1, show a concomitant lack of nk2.2 expression. Together, these results suggest a requirement of shh/vhh-1 protein for the spatial regulation of nk2.2 expression.
我们分离出了斑马鱼nk2.2，它是同源异型盒基因Nk-2家族的成员。nk2.2沿着一条边界带在连续的窄细胞带中表达，该边界带划定了大脑中最早的两个细胞核分化位置。这条细胞带位于表达信号分子音猬因子/脊椎动物刺猬因子-1（shh/vhh-1）的细胞的几个细胞直径范围内。注射shh/vhh-1 RNA会导致nk2.2的异位表达以及前脑和眼睛的伴随异常。此外，最初缺乏shh/vhh-1神经外胚层表达的独眼突变体胚胎，同时也缺乏nk2.2表达。这些结果共同表明，shh/vhh-1蛋白对于nk2.2表达的空间调控是必需的。
