Kuras L, Thomas D
Centre de Génétique Moléculaire, C.N.R.S., Gif-sur-Yvettte, France.
FEBS Lett. 1995 Jun 19;367(1):15-8. doi: 10.1016/0014-5793(95)00528-h.
The yeast Centromere binding factor I (Cbf1) belongs to the family of the DNA binding factors that recognize the consensus sequence CACGTG. Phenotypic studies of cells lacking Cbf1 revealed that this factor is actually involved in two cellular processes; the fidelity of the chromosomal segregation and the metabolism of sulfur amino acids. However, the function of Cbf1 in the regulation of the sulfur amino acid metabolism is now a matter of controversy in literature with conflicting reports about its binding to the CACGTG sequences found upstream to the methionine biosynthetic genes. To provide a reliable basis for the functional analysis of Cbf1, we present an analysis of the transcription of the methionine biosynthesic genes in cells lacking Cbf1. Our results prove that Cbf1 is indeed involved in the transcriptional regulation of the sulfur amino acid metabolism.
酵母着丝粒结合因子I（Cbf1）属于识别共有序列CACGTG的DNA结合因子家族。对缺乏Cbf1的细胞进行的表型研究表明，该因子实际上参与两个细胞过程：染色体分离的保真度和硫氨基酸的代谢。然而，Cbf1在硫氨基酸代谢调节中的功能目前在文献中存在争议，关于其与甲硫氨酸生物合成基因上游发现的CACGTG序列的结合有相互矛盾的报道。为了为Cbf1的功能分析提供可靠依据，我们对缺乏Cbf1的细胞中甲硫氨酸生物合成基因的转录进行了分析。我们的结果证明Cbf1确实参与硫氨基酸代谢的转录调控。
