Cai M, Davis R W
Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.
Cell. 1990 May 4;61(3):437-46. doi: 10.1016/0092-8674(90)90525-j.
The centromere and its binding proteins constitute the kinetochore structure of metaphase chromosomes, which is crucial for the high accuracy of the chromosome segregation process. Isolation and analysis of the gene encoding a centromere binding protein from the yeast S. cerevisiae, CBF1, are described in this paper. DNA sequence analysis of the CBF1 gene reveals homology with the transforming protein myc and a family of regulatory proteins known as the helix-loop-helix (HLH) proteins. Disruption of the CBF1 gene caused a decrease in the growth rate, an increase in the rate of chromosome loss/nondisjunction, and hypersensitivity to the antimitotic drug thiabendazole. Unexpectedly, the cbf1 null mutation concomitantly resulted in a methionine auxotrophic phenotype, which suggests that CBF1, like other HLH proteins in higher eukaryotic cells, participates in the regulation of gene expression.
着丝粒及其结合蛋白构成了中期染色体的动粒结构，这对于染色体分离过程的高度准确性至关重要。本文描述了从酿酒酵母中分离和分析编码着丝粒结合蛋白的基因CBF1。CBF1基因的DNA序列分析显示与转化蛋白myc以及一类称为螺旋-环-螺旋（HLH）蛋白的调节蛋白家族具有同源性。CBF1基因的破坏导致生长速率降低、染色体丢失/不分离率增加以及对抗有丝分裂药物噻苯咪唑过敏。出乎意料的是，cbf1缺失突变同时导致甲硫氨酸营养缺陷型表型，这表明CBF1与高等真核细胞中的其他HLH蛋白一样，参与基因表达的调控。
