Akimenko M A, Ekker M
Loeb Institute for Medical Research, Department of Medicine, University of Ottawa, Ontario, Canada.
Dev Biol. 1995 Jul;170(1):243-7. doi: 10.1006/dbio.1995.1211.
The Sonic hedgehog gene has been identified as a candidate for the signal mediating the function of the zone of polarizing activity (ZPA) during limb development in tetrapods. To better understand the early steps of development of paired fin buds in fish, we have analyzed the regulation of the zebrafish Sonic hedgehog gene (shh/vhh-1) in response to retinoic acid. Systemic administration of retinoic acid (RA) to zebrafish embryos during the initial stages of pectoral fin bud development resulted in the induction of ectopic expression of shh/vhh-1 on the anterior margin of the bud under the apical ectodermal ridge and in abnormal pectoral fin bud morphology. RA treatment also resulted in ectopic shh/vhh-1 expression in floor plate cells at the caudal end of the neural keel. These results suggest that the control of ZPA function during the initial stages of development of paired appendages has been conserved between fish and tetrapods.
音猬因子基因已被确定为四足动物肢体发育过程中介导极化活性区（ZPA）功能的信号候选基因。为了更好地理解鱼类成对鳍芽发育的早期步骤，我们分析了斑马鱼音猬因子基因（shh/vhh-1）对视黄酸的反应调控。在胸鳍芽发育的初始阶段，对斑马鱼胚胎全身施用视黄酸（RA），导致在芽的前缘、顶端外胚层嵴下方诱导shh/vhh-1的异位表达，并导致胸鳍芽形态异常。RA处理还导致神经嵴尾端的底板细胞中shh/vhh-1异位表达。这些结果表明，在鱼类和四足动物中，成对附肢发育初始阶段ZPA功能的控制是保守的。
