Isolation of 115 human chromosome 8-specific expressed-sequence tags by exon amplification.

Exon-amplification experiments were undertaken to isolate potentially transcribable sequences from cosmid clones that previously had been mapped to subchromosomal bands of human chromosome 8 by fluorescence in situ hybridization. From 253 cosmids subjected to this procedure so far, we isolated 169 fragments and confirmed that they had been derived from the original cosmid clones. Among them, 38 revealed homology to repetitive DNA sequences such as Alu and L1 elements. The other 131 were unique sequences, but of these only 115 contained discernible open reading frames. Among these 115 sequences, 15 were identical to parts of six known genes listed in the public database. On the basis of information derived from mapping the original cosmid clones, we were able to localize two of these known genes, zinc finger protein 7 and heat shock transcription factor 1, to 8q24.3. Furthermore, we have proven that some of these clones are parts of the transcribed products by an exon connection method or by isolation of a novel cDNA that is homologous to murine clathrin-associated protein. The expressed-sequence tags isolated here will be useful resources for a transcriptional map of chromosome 8 and for isolation of new genes.