Saito T, Seki N, Matsuda Y, Kitahara M, Murata M, Kanda N, Nomura N, Yamamoto T, Hori T A
Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
Genomics. 1995 Mar 20;26(2):382-4. doi: 10.1016/0888-7543(95)80224-a.
From a newly determined cDNA sequence of the human ERK gene, a highly hydrophobic portion was identified upstream of the putative tyrosine kinase domain. This is the first evidence that the ERK protein possesses a receptor-like membrane-spanning structure. Fluorescence in situ hybridization analysis of R-banded metaphase chromosomes revealed that the ERK gene is located in chromosome region 1p36.1. This locus is near the frequent translocation breakpoint or deletion region of neuroblastoma and some other cancers. A comparative mapping study of the mouse and rat homologues indicated that each counterpart maps to the mouse chromosome 4D2.2-D3 and rat chromosome 5q36.13 regions, both of which have conserved linkage homology to human chromosome 1p.
从新确定的人类ERK基因的cDNA序列中，在假定的酪氨酸激酶结构域上游鉴定出一个高度疏水的部分。这是ERK蛋白具有受体样跨膜结构的首个证据。对R带中期染色体进行的荧光原位杂交分析表明，ERK基因位于染色体区域1p36.1。该位点靠近神经母细胞瘤和其他一些癌症常见的易位断点或缺失区域。对小鼠和大鼠同源物的比较图谱研究表明，它们各自对应于小鼠染色体4D2.2 - D3和大鼠染色体5q36.13区域，这两个区域与人类染色体1p均具有保守的连锁同源性。
