25个人类X连锁基因的小鼠和大鼠同源基因的比较荧光原位杂交图谱分析。

Comparative FISH mapping of mouse and rat homologues of twenty-five human X-linked genes.

作者信息

Kuroiwa A, Watanabe T, Hishigaki H, Takahashi E, Namikawa T, Matsuda Y

机构信息

Laboratory of Animal Genetics, School of Agricultural Sciences, Nagoya University, Nagoya, Japan.

出版信息

Cytogenet Cell Genet. 1998;81(3-4):208-12. doi: 10.1159/000015032.

DOI:10.1159/000015032

PMID:9730605

Abstract

We constructed a comparative cytogenetic map of 25 functional genes in mouse and rat X chromosomes by direct R-banding fluorescence in situ hybridization. Nineteen and 22 out of the 25 genes, which have been mapped on the human X chromosome, were newly localized to mouse and rat X chromosomes, respectively. Twenty-two additional genes were integrated in the rat-mouse-human comparative map of the X chromosome in this study. Comparison of the gene order indicated the presence of four chromosome segments with conserved linkage homology between mouse and rat X chromosomes, suggesting that a minimum of four chromosomal inversion events occurred between mouse and rat X chromosomes during the evolution of the two species. Four chromosome segments with conserved linkage homology were found between human and rat X chromosomes.

摘要

我们通过直接R带荧光原位杂交构建了小鼠和大鼠X染色体上25个功能基因的比较细胞遗传学图谱。在已定位到人类X染色体上的这25个基因中，分别有19个和22个新定位到了小鼠和大鼠的X染色体上。本研究中又有22个基因整合到了大鼠-小鼠-人类X染色体比较图谱中。基因顺序的比较表明，小鼠和大鼠X染色体之间存在四个具有保守连锁同源性的染色体片段，这表明在这两个物种的进化过程中，小鼠和大鼠X染色体之间至少发生了四次染色体倒位事件。在人类和大鼠X染色体之间发现了四个具有保守连锁同源性的染色体片段。