Kishishita M, Takeda Y, Minamide W, Fujita K
College of Medical Technology, Kyoto University.
Kansenshogaku Zasshi. 1995 May;69(5):568-71. doi: 10.11150/kansenshogakuzasshi1970.69.568.
The distribution of the genes of streptococcal pyrogenic exotoxin (speA, B and C) among the 400 clinical isolates of Streptococcus pyogenes from Japanese pharnyx was detected by the PCR method (Nihonrinsho 50: 326, 1992). Sixty, 399 and 303 isolates were positive for the speA, B and C genes, respectively. However, only one isolate had none of these genes. Several isolates possessed two or three genes, i.e., both speB & C, speA & B or speA, B & C were found in 258, 30 and 30 isolates, respectively. No isolate possessing both speA & C without speB was found in this study. We concluded that the PCR method is much more useful for the epidemiological study on streptococcal pyrogenic exotoxins (SPE) of S. pyogenes isolates because of specificity, rapidity and sensitivity of this method compared with the conventional SPE identification method.
采用PCR方法检测了从日本咽炎患者中分离出的400株化脓性链球菌临床分离株中化脓性链球菌热原性外毒素基因（speA、B和C）的分布情况（《日本临床》50:326,1992）。speA、B和C基因阳性的分离株分别为60株、399株和303株。然而，只有1株分离株不携带这些基因。有几株分离株携带两个或三个基因，即分别在258株、30株和30株分离株中发现了speB和C、speA和B或speA、B和C。本研究中未发现同时携带speA和C而不携带speB的分离株。我们得出结论，与传统的SPE鉴定方法相比，由于PCR方法具有特异性、快速性和敏感性，因此在化脓性链球菌分离株的化脓性链球菌热原性外毒素（SPE）流行病学研究中更有用。
