Doi K, Ikegami H, Ishii K, Doi C, Yamanouchi Y, Sugano S
Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, Japan.
Lab Anim. 1995 Apr;29(2):180-4. doi: 10.1258/002367795780740285.
Encephalomyocarditis (EMC) virus infection in the common vole was examined for the first time. Sixteen 8-week-old males inoculated intraperitoneally with 10(5) plaque-forming units (pfu)/animal of the D variant of EMC virus were killed 3 and 7 days after inoculation (3 and 7 DAI). Viral replication was detected in the brain (10(5) pfu/g), heart (10(4) pfu/g) and pancreas (10(7) pfu/g) of all 8 animals at 3DAI. It was found in the pancreas (10(3) pfu/g) of all 8 animals and in the brain (10(4) pfu/g) of 2 of 8 animals at 7 DAI. Histopathological changes were observed in the brain (mild mononuclear cell infiltration around capillaries and sporadic pyknosis of neurons), heart (minimal myocardial necrosis) and pancreas (prominent acinar cell necrosis with inflammatory exudation) at 3DAI. At 7 DAI, replacement of necrotic tissues by mesenchymal cells and regeneration of acinar cells were conspicuous in the pancreas. Throughout the experimental period, no evidence for diabetogenic effect was found.
首次对普通田鼠感染脑心肌炎（EMC）病毒进行了研究。16只8周龄雄性田鼠腹腔注射10⁵空斑形成单位（pfu）/只动物的EMC病毒D变种，分别于接种后3天和7天（3DAI和7DAI）处死。在接种后3天，所有8只动物的脑（10⁵ pfu/g）、心脏（10⁴ pfu/g）和胰腺（10⁷ pfu/g）均检测到病毒复制。在接种后7天，所有8只动物的胰腺（10³ pfu/g）以及8只动物中的2只的脑（10⁴ pfu/g）检测到病毒。接种后3天，在脑（毛细血管周围轻度单核细胞浸润和散在神经元固缩）、心脏（轻微心肌坏死）和胰腺（腺泡细胞显著坏死伴炎性渗出）观察到组织病理学变化。接种后7天，胰腺中坏死组织被间充质细胞替代以及腺泡细胞再生明显。在整个实验期间，未发现致糖尿病作用的证据。
