Hoogstraten-Miller S, Bellinger D, Reddick R, Read M, Sigman J, Madden V
Department of Pathology, School of Medicine, University of North Carolina at Chapel Hill 27599, USA.
Lab Anim Sci. 1995 Apr;45(2):151-9.
By standard laboratory methods the presence and activity of von Willebrand factor (vWF) was detected and characterized in the ferret (Mustela putorius furo); vWF in plasma, platelets, and selected tissues (thoracic aorta, cranial vena cava, thoracic portion of caudal vena cava, and lung) was documented. Activity, antigenic concentration, plasma multimeric distribution, and localization within tissues were similar to those features in other species. Two differences were apparent: multimeric distribution of platelet vWF was skewed toward the smaller molecular weight multimers, and mucous goblet, but not ciliated, cells of the bronchial epithelium stained positive for vWF. Larger molecular weight multimers were not released subsequent to administration of 1-deamino-8-D-arginine-vasopressin. The ferret may be a useful animal model in studying the role of vWF in hemostasis, thrombosis, and atherosclerosis. In particular, the role of small molecular weight multimers found in ferret platelets may provide further insight into the roles of platelet vWF multimeric distribution, platelet adhesion, and thrombosis.
通过标准实验室方法，在雪貂（Mustela putorius furo）中检测并鉴定了血管性血友病因子（vWF）的存在和活性；记录了血浆、血小板及选定组织（胸主动脉、颅腔静脉、尾腔静脉胸段和肺）中的vWF。其活性、抗原浓度、血浆多聚体分布及在组织内的定位与其他物种的这些特征相似。有两点明显差异：血小板vWF的多聚体分布偏向分子量较小的多聚体，并且支气管上皮的黏液杯状细胞（而非纤毛细胞）vWF染色呈阳性。给予1-去氨基-8-D-精氨酸血管加压素后，未释放出分子量较大的多聚体。雪貂可能是研究vWF在止血、血栓形成和动脉粥样硬化中作用的有用动物模型。特别是，雪貂血小板中发现的小分子多聚体的作用可能会为血小板vWF多聚体分布、血小板黏附和血栓形成的作用提供进一步的见解。
