McCarroll D R, Waters D C, Steidley K R, Clift R, McDonald T P
Department of Pediatrics, University of Illinois College of Medicine, Peoria 61656.
Exp Hematol. 1988 Dec;16(11):929-37.
Washed canine platelets were shown to express a significant level of von Willebrand factor (vWf). Canine platelet vWf differed from canine plasma vWf by the absence of satellite bands associated with each multimer when resolved by SDS-agarose gel electrophoresis. Expression and multimeric composition of canine platelet vWf was quite similar to that of human platelet vWf. Quantification in both lysed, washed canine platelets and in releasate of washed canine platelets yielded estimates of platelet vWf at approximately 2% of circulating vWf in this species, with approximately 15% of this being released into the fluid phase on activation. This contrasts with findings in humans, in which approximately 10%-25% of circulating vWf is compartmentalized in platelets. The difference in relative levels of canine and human platelet vWf could not be accounted for by differences in platelet ultrastructure. The decreased relative level may account for reports that canine platelets contain no vWf.
经洗涤的犬血小板显示出表达显著水平的血管性血友病因子（vWf）。当通过SDS-琼脂糖凝胶电泳分离时，犬血小板vWf与犬血浆vWf的不同之处在于，其缺乏与每个多聚体相关的卫星带。犬血小板vWf的表达和多聚体组成与人类血小板vWf非常相似。对裂解的、经洗涤的犬血小板以及经洗涤的犬血小板释放物进行定量分析，得出该物种血小板vWf的估计值约为循环vWf的2%，其中约15%在激活时释放到液相中。这与人类的研究结果形成对比，在人类中，约10%-25%的循环vWf储存在血小板中。犬和人类血小板vWf相对水平的差异不能用血小板超微结构的差异来解释。相对水平降低可能解释了关于犬血小板不含vWf的报道。
