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用铟-111 喹啉对猪血小板进行标记的简单方法。

Simple method for labeling of porcine platelets with indium-111 oxine.

作者信息

Søfteland E, Hervig T, Framstad T, Holmsen H

机构信息

Department of Biochemistry and Molecular Biology, University of Bergen, Norway.

出版信息

Lab Anim Sci. 1995 Apr;45(2):195-8.

PMID:7603024
Abstract

A method for labeling of porcine platelets with Indium-111 oxine (8-hydroxyquinoline) is described. Mean labeling efficiency was 78.8% (+/- 8.8%, SD), platelet function remained intact as measured by aggregometry in response to acid citrate dextrose, and the procedure took an average of 159 min (2 h, 39 min), including considerable walking distances in the laboratory. A short listing of other methods for labeling of porcine platelets and platelets from other animal species is included. The described method is easy to perform, has a constantly high labeling efficiency, and is no more time-consuming than other methods.

摘要

本文描述了一种用铟-111 喹啉(8-羟基喹啉)标记猪血小板的方法。平均标记效率为 78.8%(±8.8%,标准差),通过聚集测定法检测,血小板功能在柠檬酸葡萄糖作用下保持完整,该过程平均耗时 159 分钟(2 小时 39 分钟),包括在实验室中相当长的步行距离。文中还简要列举了其他标记猪血小板及其他动物物种血小板的方法。所描述的方法易于操作,标记效率始终很高,且并不比其他方法更耗时。

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