Gardner D J, Davis J A, Weina P J, Theune B
Division of Veterinary Services, Food and Drug Administration, Bethesda, MD 20892, USA.
Lab Anim Sci. 1995 Apr;45(2):199-204.
Variation in the duration of surgical anesthesia in mice prompted an evaluation of various commonly used anesthetics. Using biotelemetric technology, we evaluated the effects of six anesthetic regimens (tribromoethanol, ketamine and acetylpromazine in combination, Telazol and xylazine in two combinations, pentobarbital, and methoxyflurane) on temperature and activity. Six groups of four male HSD:ICR mice received one of the anesthetic regimens or an equivalent volume of saline. Induction time (time from anesthetic administration until righting reflex loss) and duration of anesthesia (loss of response to interdigital toe pinch) were evaluated. Methoxyflurane and both doses of Telazol combinations resulted in the shortest and most repeatable induction times. None of the mice in the ketamine/acetylpromazine- and pentobarbital-treated groups lost the interdigital toe pinch reflex. Duration of anesthesia was superior in the two Telazol/xylazine-treated groups. A direct correlation existed between duration of anesthesia and magnitude and duration of temperature reduction. Duration of anesthesia can be used to predict extent of hypothermia.
小鼠手术麻醉持续时间的差异促使人们对各种常用麻醉剂进行评估。我们使用生物遥测技术，评估了六种麻醉方案（三溴乙醇、氯胺酮和乙酰丙嗪联合使用、两种组合的替来他明和赛拉嗪、戊巴比妥和甲氧氟烷）对体温和活动的影响。六组四只雄性HSD:ICR小鼠接受了其中一种麻醉方案或等量的生理盐水。评估了诱导时间（从给药麻醉剂到翻正反射消失的时间）和麻醉持续时间（对趾间趾捏无反应的时间）。甲氧氟烷和两种剂量的替来他明组合导致最短且最可重复的诱导时间。氯胺酮/乙酰丙嗪组和戊巴比妥处理组的小鼠均未失去趾间趾捏反射。两个替来他明/赛拉嗪处理组的麻醉持续时间更长。麻醉持续时间与体温降低的幅度和持续时间之间存在直接相关性。麻醉持续时间可用于预测体温过低的程度。
