Vallance P, Patton S, Bhagat K, MacAllister R, Radomski M, Moncada S, Malinski T
Department of Pharmacology and Clinical Pharmacology, St. George's Hospital Medical School, London, UK.
Lancet. 1995 Jul 15;346(8968):153-4. doi: 10.1016/s0140-6736(95)91211-8.
Nitric oxide is a short-lived radical involved in various biological processes. We have used an electrochemical microsensor to detect nitric oxide signals in blood vessels of healthy volunteers. The sensor was inserted into a hand vein, and the vessel was stimulated with acetylcholine or bradykinin. Dose-dependent signals were detected and were attenuated by an inhibitor of nitric oxide synthase. The results provide further evidence that endothelium-derived relaxing factor is nitric oxide and demonstrate a method for monitoring the L-arginine/nitric-oxide pathway in human beings.
一氧化氮是一种参与多种生物过程的短寿命自由基。我们使用电化学微传感器来检测健康志愿者血管中的一氧化氮信号。将传感器插入手部静脉，并用乙酰胆碱或缓激肽刺激血管。检测到剂量依赖性信号，且该信号被一氧化氮合酶抑制剂减弱。这些结果进一步证明内皮衍生舒张因子是一氧化氮，并展示了一种监测人类L-精氨酸/一氧化氮途径的方法。
