在使用人淋巴细胞的体外微核试验中，2-甲基丙烯（异丁烯）、其环氧化物2-甲基-1,2-环氧丙烷和环氧丙烷的比较致突变性。

Comparative mutagenicity of 2-methylpropene (isobutene), its epoxide 2-methyl-1,2-epoxypropane and propylene oxide in the in vitro micronucleus test using human lymphocytes.

作者信息

Jorritsma U, Cornet M, Van Hummelen P, Bolt H M, Vercruysse A, Kirsch-Volders M, Rogiers V

机构信息

Institut für Arbeitsphysiologie, Universität Dortmund, Abt. Toxikologie und Arbeitsmedizin, Germany.

出版信息

Mutagenesis. 1995 Mar;10(2):101-4. doi: 10.1093/mutage/10.2.101.

DOI:10.1093/mutage/10.2.101

PMID:7603325

Abstract

2-Methylpropene (isobutene), a gaseous compound widely used in chemical industries, is metabolized to the epoxide 2-methyl-1,2-epoxypropane. The parent compound has previously been shown to be non-mutagenic in a modified Ames test, whereas the epoxide metabolite gave a positive result. In this study, both compounds have been tested in the in vitro micronucleus test using human lymphocytes. Propylene oxide, a well known mutagenic compound, served as a positive control. It was found that 2-methylpropene had no mutagenic effect, whereas its epoxide induced a statistically significant dose-dependent increase in the number of micronuclei. The effect observed was comparable with that obtained for propylene oxide.

摘要

2-甲基丙烯（异丁烯）是一种广泛应用于化学工业的气态化合物，它会代谢生成环氧化物2-甲基-1,2-环氧丙烷。先前在改良的艾姆斯试验中已表明母体化合物无致突变性，而环氧化物代谢物则给出了阳性结果。在本研究中，这两种化合物均已使用人淋巴细胞进行了体外微核试验。环氧丙烷是一种众所周知的致突变化合物，用作阳性对照。结果发现，2-甲基丙烯没有致突变作用，而其环氧化物诱导微核数量出现具有统计学意义的剂量依赖性增加。观察到的效应与环氧丙烷所获得的效应相当。