Facer C A
Department of Haematology, London Hospital Medical College, United Kingdom.
Microbiol Immunol. 1995;39(3):207-11. doi: 10.1111/j.1348-0421.1995.tb02190.x.
Lysates of Plasmodium falciparum parasitized human erythrocytes stimulate U937 cells to secrete neopterin during a 48 hr co-culture period. Neopterin secretion by U937 cells was enhanced by the addition of human interferon gamma (IFN-gamma). Several P. falciparum antigens, 'FC27' (a synthetic 'S' antigen), Ag16 (a recombinant 'S' antigen) and Ag44/RHOP3 (a recombinant merozoite rhoptry protein), also activated U937 cells to neopterin secretion and produced a similar effect on peripheral blood mononuclear cells (PBMC) from 2 of 3 normal healthy donors cultured with the antigens for 7 days. Plasma from six Nigerian malaria patients contained high neopterin concentrations ranging from 5.06 to 14.17 ng/ml. This preliminary pilot study lends support for further investigation incorporating a larger number of malaria patients and further culture experiments with U937 cells and PBMC with the aim of defining the cause and source of the large quantities of plasma neopterin produced in this infection.
恶性疟原虫寄生的人红细胞裂解物在48小时的共培养期间刺激U937细胞分泌新蝶呤。添加人干扰素γ（IFN-γ）可增强U937细胞的新蝶呤分泌。几种恶性疟原虫抗原，“FC27”（一种合成的“S”抗原）、Ag16（一种重组“S”抗原）和Ag44/RHOP3（一种重组裂殖子动基体蛋白），也激活U937细胞分泌新蝶呤，并对3名正常健康供体中的2名与抗原培养7天的外周血单核细胞（PBMC）产生类似作用。六名尼日利亚疟疾患者的血浆中含有高浓度的新蝶呤，范围为5.06至14.17 ng/ml。这项初步的试点研究为进一步调查提供了支持，该调查将纳入更多的疟疾患者，并对U937细胞和PBMC进行进一步的培养实验，目的是确定这种感染中产生大量血浆新蝶呤的原因和来源。
