Henderson D C, Sheldon J, Riches P, Hobbs J R
Department of Immunology Charing Cross and Westminster Medical School, Westminster Hospital, London, England.
Clin Exp Immunol. 1991 Mar;83(3):479-82. doi: 10.1111/j.1365-2249.1991.tb05664.x.
The pteridine neopterin is a marker of immunological activation and has been shown to be a useful marker of graft-versus-host disease (GVHD) in bone marrow transplant patients. High levels of both neopterin and interferon-gamma (IFN-gamma) were produced in vitro during mixed lymphocyte responses, which may be considered to be a model of the primary events leading to GVHD. Neopterin was shown to be produced by monocytes in response to stimulation with IFN-gamma, but not other cytokines. However, the interleukins IL-1 alpha, IL-1 beta, IL-2, and tumour necrosis factor (TNF) alpha and beta, but not IL-6, stimulated neopterin production by unfractionated peripheral blood mononuclear cells (PBMC), and culture supernatants from PBMC stimulated with IL-1 alpha, IL-1 beta, IL-2 and IL-6, but not TNF-alpha or TNF-beta induced neopterin production following transfer to fresh monocyte cultures. It therefore appears that cytokines may generate neopterin by induction of IFN-gamma, by synergy with low levels of induced IFN-gamma, or by non-IFN-gamma-dependent mechanisms.
蝶啶新蝶呤是免疫激活的标志物，已被证明是骨髓移植患者移植物抗宿主病（GVHD）的有用标志物。在混合淋巴细胞反应过程中，体外会产生高水平的新蝶呤和干扰素-γ（IFN-γ），这可被视为导致GVHD的主要事件的模型。已表明新蝶呤是单核细胞在受到IFN-γ刺激而非其他细胞因子刺激时产生的。然而，白细胞介素IL-1α、IL-1β、IL-2以及肿瘤坏死因子（TNF）α和β（而非IL-6）可刺激未分离的外周血单个核细胞（PBMC）产生新蝶呤，并且用IL-1α、IL-1β、IL-2和IL-6刺激PBMC后得到的培养上清液（而非TNF-α或TNF-β）在转移至新鲜单核细胞培养物后可诱导新蝶呤产生。因此，细胞因子可能通过诱导IFN-γ、与低水平诱导的IFN-γ协同作用或通过非IFN-γ依赖机制来产生新蝶呤。
