Cusidó L, Pujol R, Egozcue J, Garćia M
Departament de Biologia Cel.lular i Fisiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Mutat Res. 1995 Jul;329(2):131-41. doi: 10.1016/0027-5107(95)00029-i.
The reproductive system can be especially sensitive to the toxic, carcinogenic or mutagenic effects of alkylating agents. However, since studies of such effects on germ cells are complex, their analysis has been frequently overlooked. In humans, occupational or therapeutic exposure to cyclophosphamide has been associated with male (azoospermia) and female (ovarian failure) sterility or infertility. In this work, we have studied the effect of cyclophosphamide on the formation of the synaptonemal complexes in female rat fetuses. Our results indicate that cyclophosphamide administered at 16 days of gestation, when most germ cells are in a proliferative stage in the female rat, significantly increases the frequency of synaptonemal complex and nucleolar fragmentation in a dose-dependent way.
生殖系统对烷化剂的毒性、致癌性或致突变性可能特别敏感。然而，由于对此类对生殖细胞影响的研究较为复杂，其分析常常被忽视。在人类中，职业性或治疗性接触环磷酰胺与男性（无精子症）和女性（卵巢功能衰竭）的不育或不孕有关。在这项研究中，我们研究了环磷酰胺对雌性大鼠胎儿联会复合体形成的影响。我们的结果表明，在妊娠第16天给予环磷酰胺（此时大多数生殖细胞处于雌性大鼠的增殖阶段），会以剂量依赖的方式显著增加联会复合体和核仁断裂的频率。
