Yoshihara T, Kaname H, Ishii T, Igarashi M
Department of Otolaryngology, Tokyo Women's Medical College, Japan.
ORL J Otorhinolaryngol Relat Spec. 1995 May-Jun;57(3):115-21. doi: 10.1159/000276723.
Subepithelial fiber components of the round window membrane (RWM) of the guinea pig were studied by the following methods: transmission electron microscopy (TEM), scanning electron microscopy (SEM) with NaOH treatment, high-voltage electron microscopy (HVEM) and immunohistochemical staining. SEM observation revealed intersecting collagen fibers and a vascular network in the middle layer. TEM observation showed that at the attachment region to the bone fibroblasts, collagen fibers and elastic fibers were intermingled with each other, and mesenchymal cells (bone-lining cells) were scattered on the surface of the bone. The cytoplasmic processes of fibroblasts and bone-lining cells were connected by interdigitations. HVEM demonstrated that collagen fibers of the RWM were directly linked to the fibers of the bone matrix. Immunohistochemically, the middle layer was positive for antibody to type I collagen. Immunoreaction for fibronectin was also positive at the middle layer and most intense in the region attached to the bone. Thus, the RWM is securely attached to the bone by type I collagen fibers together with the bone-lining cells and fibroblasts.
采用以下方法研究豚鼠圆窗膜（RWM）的上皮下纤维成分：透射电子显微镜（TEM）、经氢氧化钠处理的扫描电子显微镜（SEM）、高压电子显微镜（HVEM）和免疫组织化学染色。SEM观察显示中层有交叉的胶原纤维和血管网络。TEM观察表明，在与骨成纤维细胞的附着区域，胶原纤维和弹性纤维相互交织，间充质细胞（骨衬细胞）散在骨表面。成纤维细胞和骨衬细胞的胞质突起通过指状交叉相连。HVEM显示RWM的胶原纤维直接与骨基质的纤维相连。免疫组织化学检测显示，中层对I型胶原抗体呈阳性。中层对纤连蛋白的免疫反应也呈阳性，且在与骨附着的区域最为强烈。因此，RWM通过I型胶原纤维以及骨衬细胞和成纤维细胞牢固地附着于骨。
